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A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

Article DOI: 10.3791/61776-v • 14:34 min • May 6th, 2022
May 6th, 2022 •
*1, *1, *2,3, 1, 1, 1, 1, 1, 1, 1,4, 1, 5, 6, 7, 3, 1
1National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB, Shaanxi Normal University College of Life Sciences, 2Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine, Xi'an Medical University, 3Ohio State University College of Medicine, 4Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 5University of Pennsylvania ULAR, 6University of Maryland School of Medicine, 7Columbia University Medical Center
* These authors contributed equally

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요약
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Here, we describe a protocol for detection and localization of Drosophila embryo protein and RNA from collection to pre-embedding and embedding, immunostaining, and mRNA in situ hybridization.

Tags

Immunohistochemistry RNA In-situ Distribution Early Drosophila Embryo Lipid-rich Embryos Chitin-rich Chorion Mounting Embryos Glass Surfaces Histochemistry In-situ Hybridization Potassium Dichromate 95% Ethanol Chrome Alum-gelatin Gelatin Coating Slide Preparation
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