Methods Collections

Chloroplast research methods: probing the localization, topology, targeting and interactions of chloroplast proteins

Collection Overview

Chloroplasts are the organelles that define plants. Along with many other metabolic, developmental and signalling functions, chloroplasts are responsible for photosynthesis – the process whereby sunlight energy is harnessed to power the cellular activities of life. Consequently, chloroplasts are essential, not only for plants but also for the myriad ecosystems that depend on plants, and for agriculture. Chloroplasts are composed of thousands of different proteins, most of which are nucleus Show More



A simple and efficient method for studying protein import into chloroplasts using protoplasts

Inhwan Wang*1, Hangju Kang1, Junho Lee1
1Pohang University of Science and Technology, Department of Life Science

Chloroplasts are an essential organelle in plants that are responsible for various cellular processes such as photosynthesis, production of many secondary metabolites, and lipid production. For these various physiological processes, chloroplasts need a large number of proteins. Over 95% of chloroplast proteins are nucleus-encoded and imported from the cytosol after translation on the cytosolic ribosomes. Thus, proper import or targeting of these nucleus-encoded chloroplast proteins to chloroplas Show More

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