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Studying Protein Import into Chloroplasts Using Protoplasts

Studying Protein Import into Chloroplasts Using Protoplasts

Article DOI: 10.3791/58441-v 06:29 min December 10th, 2018
December 10th, 2018



Here we describe a protocol to express proteins into protoplasts by using PEG-mediated transformation method. The method provides easy expression of proteins of interest, and efficient investigation of protein localization and the import process for various experimental conditions in vivo.



Protein Import Chloroplasts Protoplasts Cell Biology En Vivo Conditions Leaf Tissues Enzyme Solution Petioles Stems Incubation Filter Sucrose Solution Centrifuge Patura Pipette W5 Solution
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