Name: operons in bacterial gene control
Uploaded: 2020-11-23T11:47:37.000Z
Duration: 2 min 9 s
Channel: JoVE

- [Verteller] In prokaryotische cellen is een operon een groep van genetische sequenties die regulatorische elementen en verschillende eiwitcoderende genen bevatten, de structurele genen die samen worden getranscribeerd. Een van de best bestudeerde gevallen is de lac-operon in bacteriën dat drie genen bevat, lac Z, lac Y, en lac A, die de enzymen coderen die nodig zijn voor het metabolisme van lactose, samen met de promotor, operator en terminator sequenties die de expressie van de lac-genen regelen. Normaal gesproken, wanneer de glucoseconcentratie overvloedig is, bindt het lac repressor-eiwit zich stevig aan de operator en verhindert het de transcriptie van de lac-genen door de binding van het RNA-polymerase aan de promotor te blokkeren.

De lac onderdrukker wordt constitutief uitgedrukt, wat betekent dat het gen dat het codeert, standaard wordt aangezet. Wanneer de glucoseconcentratie zeer laag is, zal de cel lactose als energiebron gebruiken. Eenmaal aanwezig in de cel, wordt een deel van de lactose omgezet in een aangepaste versie, allolactose genaamd, die bekend staat als de bevorderaar van de lac operon omdat het zich aan de repressor bindt en deze blokkert, wat de expressie van de lac genen veroorzaakt.

Bovendien, bij lage glucoseniveaus, neemt de hoeveelheid van de signaalmolecule cyclisch AMP toe en bindt deze zich aan het katabolische activator-proteïne, of CAP. Samen binden ze zich aan een reguleringssequentie die proximaal stroomopwaarts van de promotor ligt en helpen ze RNA-polymerase aan te trekken om de transcriptie aanzienlijk te verhogen. Tijdens de transcriptie wordt een enkele mRNA streng geproduceerd en vrijgegeven wanneer de polymerase de terminatorsequentie bereikt. Uit dit mRNA worden de drie eiwitten die nodig zijn voor de verwerking van lactose omgezet.

operons

Operons

Prokaryoten kunnen genexpressie controleren via operons – DNA-sequenties die bestaan uit regulerende elementen en geclusterde, functioneel gerelateerde eiwitcoderende genen. Operons gebruiken een enkele promotorsequentie om de transcriptie van een gencluster (dat wil zeggen een groep structurele genen) naar een enkel mRNA-molecuul te initiëren. De terminatorsequentie beëindigt de transcriptie. Een operatorsequentie, gelegen tussen de promotor en structurele genen, verbiedt de transcriptionele activiteit van het operon als het wordt gebonden door een repressoreiwit. Alles bij elkaar vormen de promotor, operator, structurele genen en terminator de kern van een operon.

Operons zijn meestal induceerbaar of onderdrukbaar. Induceerbare operons, zoals het bacteriële lacoperon, zijn normaal gesproken 'uit'. maar gaat "aan" in aanwezigheid van een klein molecuul dat een inductor wordt genoemd (bijvoorbeeld allolactose). Wanneer glucose afwezig is, maar lactose wel aanwezig is, bindt en inactiveert allolactose de lacoperon-repressor-waardoor het operon enzymen kan genereren die verantwoordelijk zijn voor het lactosemetabolisme.

Onderdrukbare operons, zoals het bacteriële trpoperon, staan meestal 'aan'. maar zal "uit" gaan in aanwezigheid van een klein molecuul dat een corepressor wordt genoemd (bijvoorbeeld tryptofaan). Wanneer tryptofaan – een essentieel aminozuur – overvloedig aanwezig is, bindt en activeert tryptofaan de trprepressor–waardoor wordt voorkomen dat het operon enzymen aanmaakt die nodig zijn voor de synthese ervan.

Operons kunnen ook constitutief (dat wil zeggen continu) actief zijn. Bacteriële ribosomaal RNA (rRNA)-operons staan bijvoorbeeld altijd 'aan'. omdat rRNA's voortdurend nodig zijn voor vertaling.

Andere regulerende elementen dragen ook bij aan de gecoördineerde genexpressie van een operon. Regulerende genen coderen voor transcriptionele activator- of repressoreiwitten. De lacI- en trpR-genen coderen bijvoorbeeld voor de repressoren van hun respectievelijke operonen. Aanvullende regulerende sequenties, zoals de catabolietactivatoreiwit (CAP)-bindingsplaats van het lac-operon, verschaffen bindingsplaatsen voor andere activatoren of repressoren. Wanneer de glucosewaarde bijvoorbeeld laag is, activeert een signaalmolecuul (dat wil zeggen cyclisch AMP) CAP, waardoor het de CAP-plaats kan binden, RNA-polymerase kan rekruteren en lac-operon-transcriptie kan initiëren.

Prokaryotes can control gene expression through operons&mdash;DNA sequences consisting of regulatory elements and clustered, functionally related ...

Education

JoVE Core

Molecular Biology

Moleculaire biologie

Unit 1

Gene Expression

Genexpressie

KEY TERMS AND CONCEPTS

BELANGRIJKE TERMEN EN CONCEPTEN

operons in bacterial gene control

Operons help bacteria control groups of genes at the same time. An operon is a set of genes that are regulated together and can be turned on or off as a unit. This shared control helps bacteria respond quickly to changes in their environment.

In many operons, a promoter gives RNA polymerase a place to bind and start transcription. The operon also includes a regulatory region that helps control whether the genes are expressed. When conditions change, the cell can use this setup to manage gene activity efficiently.

Operons are a key example of gene regulation in prokaryotes. The transcript highlights the lac operon as a model for this process. Studying operons shows how bacteria coordinate gene expression without separating the genes into different control systems.

Operons in Bacterial Gene Control

cell specific gene expression

How Cells Switch Genes On and Off

Cells switch genes on and off to make different proteins for different jobs. In multicellular organisms, many cell types are built from the same DNA, but they do not use the same genes in the same way. This difference in gene expression helps explain why cells can look and act so differently. Liver cells are a clear example. They help detoxify blood, make bile to help digest fats, and produce proteins needed for metabolism. To do these jobs, liver cells must express a specific set of genes.

regulation of expression occurs at multiple steps

regulation-of-expression-occurs-at-multiple-steps

How Cells Control Gene Expression

Cells control gene expression at several points between DNA and protein. The most common control point is transcription. At this stage, proteins bind to short regulatory DNA sequences and can either increase or reduce transcription of the linked gene. After transcription, a precursor mRNA is made. This pre-mRNA contains exons, which code for protein, and introns, which do not. It must be processed before translation, and splicing removes the introns and joins the exons. Cells can also regulate...

cis-regulatory sequences

How Cis-Regulatory DNA Controls Transcription

Cis-regulatory sequences are short, non-coding DNA segments that control gene transcription. They sit on the same chromosome as the genes they regulate and act as binding sites for transcriptional regulators. In eukaryotes, these proteins help control gene expression patterns in different cell types. These DNA sequences can be close to a gene or thousands of bases away. When they are far from the gene in the DNA sequence, they are often brought close together in space because chromosomes are...

cooperative binding of transcription regulators

cooperative-binding-of-transcription-regulators

How Transcription Factors Bind DNA

Transcriptional regulators bind to cis-regulatory DNA sequences to control gene transcription. These DNA sequences are very short, usually fewer than ten nucleotide pairs long. Because they are so short, the same sequence can appear by chance in many places across the genome. Some regulators can also bind to groups of similar sequences. That raises the chance of random binding even more. To lower this risk, transcription regulators often form dimers, which are pairs of proteins that bind a DNA...

prokaryotic transcriptional activators and repressors

prokaryotic-transcriptional-activators-and-repressors

Operon Control by Activators and Repressors

Operon control in prokaryotes depends on activators and repressors. Prokaryotic genes are often arranged in groups, not one by one like in eukaryotes. Genes that help carry out the same biochemical process are placed together with their regulatory elements. This group is called an operon, and it is transcribed into a single polycistronic mRNA strand. Activators and repressors are DNA-binding proteins that control transcription in an operon. Activators bind near the promoter, which is the site...

Operons help bacteria control groups of genes at the same time. An operon is a set of genes that are regulated together and can be turned on or off as a unit. This shared control helps bacteria respond quickly to changes in their environment. In many operons, a promoter gives RNA polymerase a place to bind and start transcription. The operon also includes a regulatory region that helps control whether the genes are expressed. When conditions change, the cell can use this setup to manage gene...

the eukaryotic promoter region

Eukaryotic Promoter Motifs and Gene Control

The eukaryotic promoter region is a stretch of DNA located upstream of a gene. It includes an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. These DNA signals help control when transcription begins. The promoter can be divided into proximal and distal regions. The proximal promoter lies close to the gene and includes cis-regulatory sequences and the core promoter. The core promoter is the RNA polymerase binding site, and it is usually found...

co-activators and co-repressors

Chromatin Control in Gene Transcription

Chromatin control plays a major role in gene transcription in eukaryotes. Gene expression is regulated by several proteins that work together at a gene regulatory site. Some of these proteins bind directly to specific DNA sequences, while co-regulators attach to those regulators but do not bind DNA on their own. Co-regulators can act as co-activators or co-repressors, depending on the complex they join. One co-regulator may even switch roles in different settings. For example, the...

eukaryotic transcription activators

How Transcription Activators Turn Genes On

Transcription activators are proteins that help turn genes on by promoting transcription from DNA to RNA. They often have two parts. One part binds to DNA, and the other part activates transcription. In some proteins, such as the glucocorticoid receptor and MyoD, one domain can do both jobs. The DNA-binding part recognizes specific regulatory sequences on the DNA. These binding domains are grouped into families based on their structure. Common examples include the leucine zipper, zinc finger,...

eukaryotic transcription inhibitors

How Eukaryotic Gene Repressors Work

Eukaryotic gene repressors help turn specific genes off when the cell needs to control growth, development, or other biochemical processes. These DNA-binding proteins regulate gene expression in eukaryotes. Their presence at the right time and place can be triggered by hormones and signals from other cells. Most transcription inhibitors have two main parts. One part binds DNA, and the other is a repressor domain, which helps shut down transcription. Repressor domains can connect with basal...

combinatorial gene control

Gene Expression by Transcription Factor Combinations

Gene expression can depend on combinations of transcription factors working together. In combinatorial gene control, several transcription factors act in a coordinated way to regulate a single gene. If one or more of these factors are missing, the level of gene expression or repression can change a lot. This kind of control helps explain how a relatively small number of transcription factors can regulate a very large number of genes. More than 30,000 genes are controlled by about 2,000 to...

induced pluripotent stem cells

Reprogramming Cells into Stem Cells

Induced pluripotent stem cells are created by reprogramming mature cells back into a stem-cell state. This process shows that specialized cells can be changed into cells with broader developmental potential. The topic is important in biology because it links cell identity, gene control, and stem cell research. These cells begin as ordinary body cells and are pushed to de-differentiate, or lose their specialized features. After reprogramming, they behave like pluripotent stem cells, which means...

master transcription regulators

MEF2C and Gene Control Cascades

MEF2C is a master transcription regulator that controls gene expression in coordinated cascades. These regulators are proteins that manage the activity of multiple genes that often work together to produce a complex outcome. When a master transcription regulator is activated, it can start a chain of transcriptional activation that supports that outcome. Master transcription regulators can act in two main ways. They may bind directly to the regulatory sequences of several genes involved in a...

epigenetic regulation

DNA Methylation and Histone Changes

Epigenetic regulation controls gene activity without changing the DNA sequence. It helps determine when genes are turned on or off in a cell. Two major epigenetic mechanisms are DNA methylation and histone modification. DNA methylation adds chemical groups to DNA, while histone changes affect how tightly DNA is packed around histone proteins. These changes can make genes easier or harder to use. In high school biology, epigenetic regulation is important for understanding how cells with the...

genomic imprinting and inheritance

Genomic Imprinting in Human Disease

Genomic imprinting affects how some inherited genes are turned on or off in human cells. Diploid organisms inherit chromosomes from both parents, and each gene usually has two alleles. In most cases, both alleles are expressed. If one allele is missing or mutated, the other can often compensate, but some genes do not work that way. In genomic imprinting, a gene or its regulatory sequence carries chemical tags, such as methyl groups, on the copy inherited from only one parent. These tags are...

key terms and concepts

Learn how operons let bacteria regulate groups of genes together, with a focus on promoters, regulatory regions, and the lac operon model.

Operons in Bacterial Gene Control | JoVE K12

molecular biology

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Operons in Bacterial Gene Control

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