Fiber Optic Bundle
Fiber Optic Bundle - Related Videos
08:50
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
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Cited by 4 •
2013
We describe a procedure for profiling salivary proteins using multiplexed microsphere-based antibody arrays. Monoclonal antibodies were covalently linked to fluorescent dye-encoded 4.5 μm polymer microspheres using carbodiimide chemistry. The modified microspheres were deposited in fiber-optic microwells to measure protein levels in saliva using fluorescence sandwich immunoassays.
13:49
High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
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Cited by 84 •
2011
In many biological and clinical situations it is advantageous to study cellular processes as they evolve in their native microenvironment. Here we describe the assembly and use of a low-cost fiber-optic microscope which can provide real time imaging in cell culture, animal studies, and clinical patient studies.
06:08
Preparation and Immunofluorescence Staining of Bundles and Single Fiber Cells from the Cortex and Nucleus of the Eye Lens
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Cited by 7 •
2023
This protocol describes methods to prepare peripheral, mature, and nuclear eye lens fiber cells for immunofluorescence staining to study complex cell-to-cell interdigitations and the membrane architecture.
10:35
Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
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2016
The assembly and use of a multimodal microendoscope is described which can co-register superficial tissue image data with tissue physiological parameters including hemoglobin concentration, melanin concentration, and oxygen saturation. This technique can be useful for evaluating tissue structure and perfusion, and can be optimized for individual needs of the investigator.
10:21
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
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Cited by 18 •
2016
Multicolor fluorescence detection in droplet microfluidics typically involves bulky and complex epifluorescence microscope-based detection systems. Here we describe a compact and modular multicolor detection scheme that utilizes an array of optical fibers to temporally encode multicolor data collected by a single photodetector.
FAQs
Each fiber guides light through its core by total internal reflection, allowing illumination or image information to travel between separated locations. This optical guidance is important when the bundle must reach a confined biological environment from a remote position, because the transmitted light does not require a direct line of sight between the source and the observation site.
Maintaining the relative position of individual fibers allows spatial information from the observed region to remain organized as it travels through the bundle. That arrangement supports image transmission rather than only undifferentiated illumination, making the bundle useful for viewing biological structures during imaging procedures in locations that are difficult to access directly.
A small diameter can help the bundle enter confined biological spaces, while flexibility supports positioning along less accessible paths. Together, these features can reduce the need for large openings and allow remote access to living systems. The resulting design is especially relevant when researchers need to observe or illuminate tissue without exposing the entire region.
For imaging, the bundle transfers organized optical information so researchers can visualize a biological environment or detect fluorescence. For targeted light delivery, the emphasis is directing illumination to a selected location, as in phototherapy. The same compact, remote-access architecture therefore supports both observation and intervention, but the desired optical outcome differs.
During minimally invasive endoscopy, the bundle provides a route for light or image information between equipment outside the body and a confined biological site. Its small diameter and flexibility help researchers access living systems through limited openings, while remote operation reduces dependence on direct visual access to the tissue being examined.
In fluorescence imaging, a bundle can support visualization of fluorescence from a biological region that is difficult to reach directly. This capability contributes to tissue monitoring by enabling optical information to be accessed remotely. The approach is valuable when researchers want to study living systems while limiting the size of the access opening.
Phototherapy applications require light to reach a selected biological region, and a fiber optic bundle can provide targeted delivery through a compact, flexible path. Remote placement helps researchers illuminate confined tissue without requiring direct line of sight. This makes the technology relevant to bioengineering studies that combine controlled light exposure with access to living systems.