M30 Elisa Apoptosis
M30 Elisa Apoptosis - Related Videos
10:06
The ELISA Method
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2023
An enzyme-linked immunosorbent assay (ELISA) is typically performed to detect the presence and/or amount of a target protein of interest within an experimental sample. Detection of the target protein is made possible by antibodies, which make the ELISA an immunoassay. Through a series of incubation and washing steps, these antibodies, which are frequently linked, or conjugated, to an enzyme, will detect protein coating the bottom of a well on a microtiter plate. When exposed to a substrate,...
14:22
ELISA Assays: Indirect, Sandwich, and Competitive
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2023
Source: Whitney Swanson1,2, Frances V. Sjaastad2,3, and Thomas S. Griffith1,2,3,4 1 Department of Urology, University of Minnesota, Minneapolis, MN 55455 2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 3 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455 4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 Enzyme-linked immunosorbent assay (ELISA) is frequently used to measure the presence...
07:42
Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
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Cited by 15 •
2011
In this protocol, we describe the direct cytoplasmic microinjection of cytochrome c protein into fibroblasts and primary sympathetic neurons. This technique allows for the introduction of cytochrome c protein into the cytoplasm of cells and mimics the release of cytochrome c from mitochondria, which occurs during apoptosis.
01:30
Apoptosis
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2023
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size reduction of the tissue.
03:55
ELISA-Based Method to Analyze Interactions between Bait and Prey Proteins
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2025
This video describes an ELISA-based technique that detects protein-protein interactions between two proteins by measuring the binding of the second protein to the immobilized first protein. The bound second protein is detected using specific antibodies labeled with an enzyme, such as horseradish peroxidase.
FAQs
The key mechanistic event is caspase cleavage of cytokeratin 18. This processing exposes a neo-epitope, meaning a newly revealed antibody-recognition site that is absent from the uncleaved protein. M30 binding therefore connects the measured signal to caspase-associated apoptotic processing, rather than simply indicating that cytokeratin 18 is present in a sample.
The M30 antibody recognizes the neo-epitope generated after caspase cleavage, not merely the full-length cytokeratin 18. This distinction gives the assay greater relevance to apoptotic epithelial-cell death than a measurement of total cytokeratin 18. It also helps separate apoptotic processing from simple protein abundance when interpreting results.
An increased M30 signal should be interpreted as evidence of greater caspase-cleaved cytokeratin 18 associated with apoptosis in the tested material. It is not, by itself, a complete measure of overall cell viability or every form of cell death. Pairing it with other cell-death or viability assays provides a broader assessment of cellular state.
The enzyme-linked detection system translates antibody binding into a measurable signal, making the molecular event suitable for quantitative comparison between samples or conditions. This readout is useful when a bioengineering study needs to track changes in apoptosis, rather than relying only on qualitative observations of tissue, culture, or organoid appearance.
A basic assay workflow centers on detecting the exposed neo-epitope in a sample, allowing M30 antibody binding, and using the enzyme-linked detection system to generate a measurable signal. The resulting value can then be compared across engineered-tissue, biomaterial-culture, or organoid conditions to evaluate differences in apoptotic cell death.
In bioengineering, M30 ELISA apoptosis measurements can be applied to engineered tissues, biomaterial cultures, and organoid models. The assay provides a quantitative endpoint for evaluating apoptotic cell death in these systems, helping investigators assess how engineered environments relate to epithelial-cell survival and how cellular outcomes change between experimental conditions.
Drug screening and process optimization are important use cases because the assay supplies a quantitative apoptosis-related endpoint. Investigators can compare conditions using the M30 signal and then interpret those results alongside viability or other cell-death measurements. This combined approach helps distinguish a change in apoptotic response from a broader change in cellular performance.