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Chapters
- 00:05Title
- 01:27Preparation of Protein Mixtures for Injection
- 03:11Cytoplasmic Microinjection of Cytochrome c
- 06:34Cytochrome c Injected Fibroblasts and Neurons
- 07:24Conclusion
In this protocol, we describe the direct cytoplasmic microinjection of cytochrome c protein into fibroblasts and primary sympathetic neurons. This technique allows for the introduction of cytochrome c protein into the cytoplasm of cells and mimics the release of cytochrome c from mitochondria, which occurs during apoptosis.
Tags
ApoptosisProgrammed Cell DeathCytoplasmic MicroinjectionCytochrome CMitochondrial Intermembrane SpaceCaspasesCytotoxic StressesSignaling CascadesHolocytochrome CHeme-attached ProteinPost-mitochondrial RegulationCDNA ConstructsCytosolic CaspasesPurified Heme-attached Cytochrome C ProteinMitochondrial Cytochrome C Release
