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Biology

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Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
 

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Article DOI: 10.3791/2773
June 29th, 2011

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Summary June 29th, 2011

In this protocol, we describe the direct cytoplasmic microinjection of cytochrome c protein into fibroblasts and primary sympathetic neurons. This technique allows for the introduction of cytochrome c protein into the cytoplasm of cells and mimics the release of cytochrome c from mitochondria, which occurs during apoptosis.

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