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June 29, 2011
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In this protocol, we describe the direct cytoplasmic microinjection of cytochrome c protein into fibroblasts and primary sympathetic neurons. This technique allows for the introduction of cytochrome c protein into the cytoplasm of cells and mimics the release of cytochrome c from mitochondria, which occurs during apoptosis.
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Cite this Article
Kole, A. J., Knight, E. R., Deshmukh, M. Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c. J. Vis. Exp. (52), e2773, doi:10.3791/2773 (2011).
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