Near Infrared Photoactivation
Near Infrared Photoactivation - Related Videos
11:20
An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
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Cited by 1 •
2017
In this protocol, caged protein kinase A (PKA), a cellular signal transduction bioeffector, was immobilized on a nanoparticle surface, microinjected into the cytosol, and activated by the upconverted UV light from near-infrared (NIR) irradiation, inducing downstream stress fiber disintegration in the cytosol.
05:02
Bimolecular Fluorescence Complementation-Coupled Photoactivated Localization Microscopy
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2025
This video describes BiFC-PALM ― a combination of photoactivated localization microscopy and bimolecular fluorescence complementation ― to assess protein-protein interactions. BiFC involves the fusion of two fluorescent protein fragments with two interacting proteins of interest. When the two proteins interact, the fragments are brought into proximity, which allows the two parts to come together and reconstitute a functional fluorescent protein. The fluorescence of a single fluorophore is...
04:33
Fluorescence Photoactivation Imaging of Neurofilament Transport in Myelinated Mouse Axons
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2025
Source: Boyer, N. P. et. al., Imaging and Analysis of Neurofilament Transport in Excised Mouse Tibial Nerve. J. Vis. Exp. (2020) This video demonstrates the fluorescence photoactivation method to analyze neurofilament transport in myelinated axons of peripheral nerves. By tracking photoactivated green fluorophore-tagged neurofilaments, the technique provides insights into axonal transport in neurodegenerative diseases.
08:11
Infrared Spectroscopy
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2023
Source: Vy M. Dong and Zhiwei Chen, Department of Chemistry, University of California, Irvine, CA This experiment will demonstrate the use of infrared (IR) spectroscopy (also known as vibrational spectroscopy) to elucidate the identity of an unknown compound by identifying the functional group(s) present. IR spectra will be obtained on an IR spectrometer using the attenuated total reflection (ATR) sampling technique with a neat sample of the unknown.
09:38
Characterizing Far-infrared Laser Emissions and the Measurement of Their Frequencies
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Cited by 4 •
2015
We describe the generation of far-infrared radiation using an optically pumped molecular laser along with the measurement of their frequencies with heterodyne techniques. The experimental system and techniques are demonstrated using difluoromethane (CH2F2) as the laser medium whose results include three new laser emissions and eight measured laser frequencies.
FAQs
Activation begins with the target material absorbing near infrared light and converting that energy into a usable trigger. The resulting response depends on how efficiently the material performs this conversion and on the type of light-sensitive system involved. Thus, material selection links the incoming wavelength to the intended chemical, physical, or biological outcome.
Near infrared penetration is important because it can allow activation at locations that visible light may not reach as effectively within tissue. In bioengineering, this property supports control of internal targets while limiting the need for direct exposure at the surface. The resulting advantage is spatially targeted intervention for living systems and device-tissue interactions.
Photothermal agents, photoswitchable molecules, and engineered biomaterials provide different routes to activation. Photothermal agents respond by converting light energy into heat-related effects, while photoswitchable molecules use light to alter their state; engineered biomaterials can translate illumination into a designed material response. These distinctions help match the component to drug release, biomolecular control, cell behavior, or tissue interaction.
Spatiotemporal control means selecting both where and when a response occurs. Near infrared photoactivation can support this control by directing illumination toward a chosen location and applying it at a selected time, while the light-sensitive material determines the resulting response. This combination is relevant when researchers need localized changes in drug release, biomolecular activity, cell behavior, or tissue interactions.
A bioengineering strategy first matches a light-sensitive material to the intended chemical, physical, or biological response. Near infrared illumination is then used to trigger that material at a selected location and time. The design goal is to connect controlled light exposure with a specific outcome, such as releasing a drug, changing biomolecular activity, or influencing interactions between cells and tissues.
Researchers may consider this approach when they need minimally invasive control over biological or material behavior. Potential bioengineering uses include triggering drug release, regulating biomolecular activity, influencing cell behavior, and directing tissue interactions. Near infrared photoactivation also relates to advanced biomedical devices, where light-responsive behavior may be incorporated into interactions with living tissue.