Optical Breadboard Assembly
Optical Breadboard Assembly - Related Videos
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
07:20
Trapping of Micro Particles in Nanoplasmonic Optical Lattice
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2017
We describe a procedure to optically trap micro-particles in nanoplasmonic optical lattice.
12:27
Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
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Cited by 3 •
2009
The assembly of a nearfield infrared microscope for imaging protein aggregates is described.
08:31
Three-dimensional Optical-resolution Photoacoustic Microscopy
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Cited by 27 •
2011
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technology capable of imaging optical absorption contrasts in vivo with cellular resolution and sensitivity. Here, we provide a visualized instruction on the experimental protocols of OR-PAM, including system configuration, system alignment, typical in vivo experimental procedures, and functional imaging schemes.
02:41
Protein Complex Assembly
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2020
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes. Many viruses self-assemble into a fully functional unit using the infected host cell to...
FAQs
Rigid construction and vibration resistance limit unwanted movement of mounted components, while the patterned mounting grid and threaded-hole array support controlled placement. Keeping lasers, lenses, mirrors, and detectors fixed helps preserve the intended optical path during measurement. This stability is particularly important when bioengineering experiments must detect small changes or maintain consistent interaction with a biological sample.
They provide repeatable attachment points for positioning optical and biological measurement hardware across the platform. Components can be secured rather than left vulnerable to shifting, making it easier to establish relationships among the light source, optical elements, detector, and sample-related hardware. The organized layout also supports integration with fluidic, mechanical, and electronic devices in a single experiment.
Component placement, mounting stability, and alignment are central factors because small positional changes can alter the optical path. Environmental vibration is another concern: movement can introduce variability even when the optical components themselves are functioning correctly. Addressing these factors helps measurements remain reproducible and reduces the risk that observed differences arise from the setup rather than from the biological system.
Even small alignment errors can change the intended optical path and interfere with measurements in microscopy, spectroscopy, biosensing, or optical manipulation. Careful positioning and secure mounting help preserve the relationship between optical components and the sample. This matters when protecting delicate samples and when distinguishing genuine biological results from variability introduced by the instrument.
The experimenter can plan positions for lasers, lenses, mirrors, detectors, and biological measurement hardware on the patterned platform. Components are then secured through the threaded mounting locations, followed by alignment of the optical path and integration with fluidic, mechanical, or electronic devices. This arrangement supports stable operation and repeatable measurements.
It supports microscopy, spectroscopy, biosensing, and optical manipulation setups. In each case, the platform provides a stable basis for coordinating light-handling components with biological measurement hardware. Its value extends beyond support: careful assembly can improve reproducibility, preserve optical alignment, and help protect delicate samples while the system interacts with biological materials.
Reliable performance is reflected in a stable optical path, consistent measurements, and improved reproducibility across experimental runs. Secure placement also helps prevent alignment-related variation from being mistaken for a biological effect. For integrated systems, successful assembly means optical components can operate alongside fluidic, mechanical, and electronic devices without compromising the intended measurement arrangement.