Laboratory Colony
Laboratory Colony - Related Videos
07:23
Inducing Polyp Bail-out in Coral Colonies to Obtain Individualized Micropropagates for Laboratory Experimental Use
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Cited by 3 •
2022
Polyp bail-out is a process induced by acute stress, in which coral polyps digest the tissue connecting them to their colony and detach from it to live as individuals. The present protocol describes how to induce coral micropropagation by bail-out using hypersaline or calcium-free seawater treatments.
06:44
Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies
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2023
In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.
09:04
Yeast Colony Embedding Method
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Cited by 7 •
2011
A method for embedding yeast colonies allowing sectioning for light and electron microscopy. This protocol allows determination of the distribution of sporulated cells and pseudohyphal cells within colonies providing a new tool toward understanding the organization of cell types within a fungal community.
18:00
Aseptic Laboratory Techniques: Plating Methods
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Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
09:01
Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory
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Cited by 4 •
2016
We present a simple method to construct 3D nematode cultivation systems called NGT-3D and NGB-3D. These can be used to study nematode fitness and behaviors in habitats that are more similar to natural Caenorhabditis elegans habitats than the standard 2D laboratory C. elegans culture plates.
FAQs
Standardized housing, nutrition, environmental conditions, and health monitoring reduce variation that could otherwise obscure experimental effects. When these factors remain consistent, researchers can more confidently associate differences in development, behavior, disease, or treatment response with the variable under investigation. This control also strengthens comparisons between experiments and supports more reproducible biological findings over time.
Breeding records help researchers track reproduction and preserve biological traits that matter to the study. They provide continuity across generations, show how the colony is being reproduced, and support decisions intended to maintain relevant characteristics. Accurate records therefore connect colony management with experimental reliability, especially when researchers need a continuing supply of organisms with consistent biological backgrounds.
Outcomes depend on how well researchers manage breeding or propagation, housing, nutrition, environmental conditions, and health. Changes in any of these factors can alter organism viability or introduce variation among individuals. Monitoring them allows researchers to distinguish effects associated with genetics, development, behavior, disease, treatments, or environmental changes from effects caused by inconsistent colony conditions.
Reproducibility improves when a colony is managed with consistent conditions, documented breeding or propagation, and ongoing health monitoring. These practices help maintain relevant traits and reduce uncontrolled differences among organisms used at different times. As a result, researchers can compare findings across experiments more effectively and interpret whether an observed response reflects the research question rather than colony variation.
Sustaining the colony requires organizing breeding or propagation, providing suitable housing and nutrition, controlling environmental conditions, and monitoring health. Researchers also maintain records that document colony continuity and relevant traits. Together, these activities keep organisms viable and available for study while helping identify management changes that could affect later experiments or the interpretation of their results.
Researchers use this approach when a study requires organisms over time under conditions they can manage and document. It is useful for investigating genetics, development, behavior, disease, treatment responses, or reactions to environmental changes. Maintaining the population also provides an ongoing source of organisms, which supports repeated experiments and comparisons rather than relying on a single observation.
Experiments can reveal how biological traits relate to genetics, development, behavior, disease, treatments, or environmental changes. Because the organisms are maintained under documented conditions, researchers can compare responses while limiting some sources of variation. The resulting observations may help connect controlled changes in the study environment with differences in organism viability or other measured biological outcomes.