Shear Flow Assay
Shear Flow Assay - Related Videos
09:20
The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
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Cited by 10 •
2016
Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.
07:40
Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
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Cited by 3 •
2016
This flow adhesion assay provides a simple, high impact model of T cell-epithelial cell interactions. A syringe pump is used to generate shear stress, and confocal microscopy captures images for quantification. The goal of these studies is to effectively quantify T cell adhesion using flow conditions.
12:43
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
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Cited by 51 •
2012
We are describing a method to subject adherent cells to laminar flow shear stress in a sterile continuous flow circuit. The cells' adhesion, morphology can be studied through the transparent chamber, samples obtained from the circuit for metabolite analysis and cells harvested after shear exposure for future experiments or culture.
11:38
A Flow Adhesion Assay to Study Leucocyte Recruitment to Human Hepatic Sinusoidal Endothelium Under Conditions of Shear Stress
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Cited by 31 •
2014
Leucocyte recruitment to the liver occurs within the specialized channels of the hepatic sinusoids which are lined by unique hepatic sinusoidal endothelial cells. Phase contrast microscopy of leucocyte recruitment across human hepatic sinusoidal endothelium under conditions of physiological shear stress can facilitate the elucidation of the molecular mechanisms which underlie this process.
12:42
Human Neutrophil Flow Chamber Adhesion Assay
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Cited by 12 •
2014
A method of quantitating neutrophil adhesion is reported. This method creates a dynamic flow environment similar to that encountered in a blood vessel. It allows the investigation of neutrophil adhesion to either purified adhesion molecules (ligand) or endothelial cell substrate (HUVEC) in a context similar to the in vivo environment with sheer stress.
FAQs
Shear stress can alter several measurable cell behaviors, including adhesion, cell shape, signaling, and gene expression. Because the fluid movement is controlled, researchers can relate these responses to the mechanical force applied at the cell surface rather than simply noting that flow occurred. This makes the assay useful for connecting physical conditions with changes in cellular function.
A chamber or microfluidic channel directs fluid across the cell or tissue surface and creates a defined shear stress. This controlled physical arrangement gives the experiment a repeatable mechanical input, allowing observed differences in adhesion, shape, signaling, or gene expression to be interpreted in relation to fluid movement. The setup therefore links force exposure with specific biological readouts.
Measurements can target both rapid and broader cellular responses. Researchers may examine whether cells remain attached, change shape, activate signaling, or alter gene expression after exposure to controlled flow. Selecting among these readouts helps match the assay to the biological question, such as a physical response at the cell surface or a more sustained change in cell state.
A typical workflow places cells or tissues in a chamber or microfluidic channel, introduces fluid movement, and exposes the biological material to the selected flow condition. Researchers then assess responses such as adhesion, shape changes, signaling, or gene expression. The sequence separates force application from outcome measurement, making it possible to examine how cells respond to controlled mechanical exposure.
Endothelial cells are a major application because the assay can examine them under blood-flow conditions relevant to the vascular environment. Observing their adhesion, shape, signaling, or gene-expression responses helps researchers investigate how physical forces relate to vascular function. The same context supports studies of processes associated with inflammation and thrombosis, as indicated by changes under controlled flow.
Beyond endothelial studies, the technique can examine leukocyte attachment and interactions between cells and biomaterials under fluid movement. These applications extend the assay from cell-intrinsic responses to behaviors at biological or material interfaces. Results can help connect the way cells adhere or respond during flow with broader questions about inflammation, thrombosis, and disease mechanisms.