Shewanella Oneidensis
Shewanella Oneidensis - Related Videos
09:56
In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS
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Cited by 8 •
2017
This article presents a method for growing a biofilm for in situ time-of-flight secondary ion mass spectrometry for chemical mapping in its hydrated state, enabled by a microfluidic reactor, System for Analysis at the liquid Vacuum Interface. The Shewanella oneidensis MR-1 with green fluorescence protein was used as a model.
09:00
Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1
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Cited by 8 •
2018
Here we present a protocol of whole-cell electrochemical experiments to study the contribution of proton transport to the rate of extracellular electron transport via the outer-membrane cytochromes complex in Shewanella oneidensis MR-1.
02:31
Measuring the Kinetic Isotope Effect on Microbial Electron Transport Using Deuterium Oxide
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2025
Source: Tokunou, Y., et al, Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1. J. Vis. Exp. (2018)This video demonstrates a whole-cell electrochemical method to assess how proton transfer affects extracellular electron transport (EET) in electroactive bacteria. By replacing protons with deuterons using deuterium oxide, the method measures changes in current to reveal a kinetic isotope effect, highlighting the role of...
FAQs
When oxygen becomes limited, Shewanella oneidensis can route electrons from organic substrates through inner-membrane and periplasmic cytochromes toward external electron acceptors. The Mtr pathway supports this transfer beyond the cell, allowing minerals or electrodes to receive electrons. This links intracellular metabolism with extracellular respiration and helps explain the organism’s adaptation to changing environmental conditions.
Cytochromes provide successive components of the electron-transfer route, while the Mtr pathway connects cellular electron flow with an external mineral or electrode. Their organization across the inner membrane and periplasm enables electrons derived from organic substrates to move outward. Studying these components helps researchers relate specific cellular machinery to extracellular electron transfer.
Oxygen availability determines which respiratory strategy is most relevant to the cells. Under oxygen-limited conditions, researchers can examine how electrons move from organic substrates through cytochromes and the Mtr pathway toward external acceptors. This makes oxygen conditions central to investigations of environmental adaptation, mineral interactions, and electron transfer to conductive surfaces.
Researchers can connect genetic analysis with conditions that vary oxygen availability, organic substrates, and the presence of external minerals or electrodes. Comparing cellular behavior under these conditions helps reveal how electron flow responds to environmental changes. Because the organism has experimentally tractable genetics, investigators can relate cellular components to respiration and extracellular electron-transfer outcomes.
Shewanella oneidensis supports studies of microbial respiration, environmental adaptation, and interactions with metals or conductive surfaces. Its extracellular electron-transfer capacity also provides a foundation for research in bioremediation, bioelectrochemical systems, and microbial fuel cells. These applications use the connection between microbial metabolism and electron movement outside the cell.
Its metabolism provides a tractable way to study how cellular electron flow affects interactions with external materials. Electrons originating from organic substrates can reach minerals or electrodes, linking microbial respiration to environmental energy transformations. Genetic analysis further helps researchers identify how cellular processes contribute to metal interactions, conductive-surface behavior, and broader energy cycling.