Acid Induced Assembly
Acid Induced Assembly - Related Videos
05:08
Solubility of Hydrophobic Compounds in Aqueous Solution Using Combinations of Self-assembling Peptide and Amino Acid
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Cited by 4 •
2017
This protocol describes a clinically-applicable means of dissolving hydrophobic compounds in an aqueous environment using combinations of self-assembling peptide and amino acid solutions. Our method resolves a major limitation of hydrophobic therapeutics, which lack safe, efficient means of solubility and delivery methods into clinical settings.
09:51
Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin
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Cited by 43 •
2009
We demonstrate the use of a constant-force extensometer to measure long-term extension (creep) of plant cell wall specimens induced by acidic buffers and expansin protein.
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
04:02
Retinoic Acid-Induced Neurogenesis using Mouse Embryonic Carcinoma Cells
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2025
This video demonstrates a simple technique for retinoic acid-induced neurogenesis in P19 mouse embryonic carcinoma cells. Retinoic acid activates transcription factors and triggers gene expression for neuronal differentiation.
02:41
Protein Complex Assembly
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2020
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes. Many viruses self-assemble into a fully functional unit using the infected host cell to...
FAQs
An acid changes the protonation state of functional groups on the building blocks. That change can modify charge, hydrogen-bonding ability, solubility, or molecular shape. As a result, interactions that previously favored dispersion or separation may instead favor association, allowing molecules, polymers, or nanoscale components to form larger structures under selected chemical conditions.
The outcome reflects the balance among electrostatic interactions, hydrogen bonding, solubility, and molecular shape. Protonation may reduce repulsion, strengthen specific hydrogen-bonding patterns, or alter how readily a component remains dissolved. Depending on the building block and conditions, these changes can favor discrete aggregates, extended networks, gels, capsules, or other organized structures.
Reversibility arises because acidity directly controls protonation equilibria rather than permanently changing the building blocks. Adjusting the acid level can restore a different charge state, hydrogen-bonding pattern, solubility, or molecular shape. The altered interaction balance may then destabilize the assembled structure or shift it toward another organization, supporting chemically responsive and adaptive materials.
Their size, connectivity, and available reactive groups influence how protonation changes organization. Individual molecules may form discrete supramolecular structures, while polymers can connect multiple interactions into extended networks. Nanoscale building blocks may generate larger organized architectures. In each case, the acid response depends on how the altered properties propagate through the component's structure.
Researchers need to define the acidity conditions that produce the desired structural change and maintain them consistently during assembly. They also select suitable molecules, polymers, or nanoscale building blocks whose protonation-sensitive groups can alter interactions or solubility. Comparing structures before and after the acid stimulus helps determine whether aggregation, network formation, or another outcome occurred.
The approach is useful when a material must respond to a controllable chemical signal. Adjusting acidity can trigger or reverse organization in supramolecular structures, gels, capsules, and related systems. This behavior supports the design of adaptive materials and provides a way to regulate structure in studies of molecular recognition, sensing, drug delivery, and catalysis.