Murine Lung Mscs
Murine Lung Mscs - Related Videos
08:18
Isolation and Enrichment of Rat Mesenchymal Stem Cells (MSCs) and Separation of Single-colony Derived MSCs
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Cited by 107 •
2010
Rat MSCs were isolated from femurs and tibias and then enriched by magnetic cell sorting. Sorted cells were confirmed for the expression of surface markers by flow cytometry. These cells were also cultured at clonal density to form single colonies and then these colonies were separated by cloning cylinders.
06:20
Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)
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Cited by 13 •
2015
The immunomodulatory properties of human mesenchymal stem cells (MSC) appear increasingly relevant for clinical application. Using a co-culture system of MSCs and peripheral blood leukocytes pre-stained with the fluorescent dye carboxyfluorescein succinimidyl ester (CFSE), we describe the in vitro assessment of MSC immunomodulation on effector leukocyte proliferation and specific subpopulations.
09:47
Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
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Cited by 3 •
2016
This protocol describes an isolation technique for obtaining primary lung resident mesenchymal stromal cells from rats, through the use of enzymatic digestion, density gradient separation, plastic adherence and CD146+ magnetic bead selection.
08:40
Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract
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Cited by 44 •
2015
The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.
05:54
An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs
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2025
This video demonstrates an in vitro technique to identify immune cells residing in murine lungs. After isolating target immune cells from murine lung tissue, they are surface-labeled using a cocktail of fluorophore-tagged antibodies against specific markers on innate and adaptive immune cells. Additionally, monocytes, macrophages, and dendritic cells are labeled using an intracellular marker. The various immune cell types are then identified using flow cytometry.
FAQs
These stromal cells act through two complementary routes: they produce extracellular matrix that helps organize the local tissue environment, and they release paracrine signals that affect nearby epithelial and vascular cells. Together, these interactions provide a mechanism for coordinating mesenchymal behavior with developing lung structures, making the cells useful for studying communication within the tissue rather than isolated cell activity.
Mesenchymal signals can shape epithelial behavior while extracellular matrix provides structural support around developing lung regions. Examining this relationship helps developmental biologists connect stromal activity with alveolar formation, rather than treating epithelial development as an autonomous process. It also offers a framework for asking how altered mesenchymal support could influence repair or remodeling after injury.
Assessment combines phenotype and function. Investigators examine characteristic marker profiles in the expanded culture and test whether the cells display mesenchymal differentiation capacity. Using both types of evidence is important because adherence and expansion describe how cells were enriched, whereas marker patterns and differentiation behavior provide additional support for identifying the resulting preparation as a mesenchymal stromal population.
A typical workflow begins by dissociating mouse lung tissue, placing the resulting cells in culture, and using adherence to enrich the stromal population. The adherent cells are then expanded before characterization. Investigators can evaluate the expanded preparation through marker profiles and mesenchymal differentiation capacity, linking the isolation procedure to biological identity.
Culture provides an experimentally accessible way to enrich and expand the population, whereas in vivo studies address its relationships within the lung’s connective-tissue compartment. The first setting supports characterization through markers and differentiation capacity; the second preserves interactions involving extracellular matrix, paracrine signaling, epithelial cells, and vascular cells. Comparing both perspectives helps relate cell properties to tissue function.
They are informative when the research question concerns how stromal populations shape lung development or respond to tissue disruption. Applications described for these cells include studying mesenchyme-epithelium interactions, alveolar formation, injury responses, and fibrotic remodeling. This range connects normal developmental processes with reparative and remodeling changes in the same organ system.
Experiments can clarify how mesenchymal cells contribute to tissue organization, repair, and homeostasis, and how their matrix production or paracrine activity affects neighboring cell populations. In developmental studies, these findings can reveal how stromal compartments support alveolar formation. In regenerative research, the same evidence may help frame questions about restoring lung structure after damage.