Saltwater Rotifers

Saltwater rotifers are microscopic marine and brackish-water animals whose rapid reproduction and transparent bodies make them useful for studying animal development. Many species alternate between parthenogenetic reproduction, in which females produce offspring without fertilization, and sexual reproduction triggered by environmental conditions, producing resistant eggs that can survive unfavorable periods. Their embryos and life-history transitions allow researchers to examine cell division, differentiation, hatching, and developmental responses to salinity, temperature, and nutrition. In developmental biology, saltwater rotifers also serve as practical live feed for cultured fish and invertebrate larvae, linking developmental research with aquaculture and studies of environmental effects on early life stages.

Saltwater Rotifers - Related Videos

Research

JoVE Journal - Biology

Measurement of Survival Time in Brachionus Rotifers: Synchronization of Maternal Conditions

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Cited by 6 •

2016

Rotifers are microscopic zooplankton used as models in ecotoxicological and aging studies. Here we provide a protocol for powerful and reproducible measurement of survival time in Brachionus rotifers. Synchronization of culture conditions over several generations is of particular importance because maternal condition affects life history of offspring.

The Complete and Updated "Rotifer Polyculture Method" for Rearing First Feeding Zebrafish

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Cited by 20 •

2016

Larval zebrafish are adapted to feed on zooplankton. It is possible to capitalize on this natural feature in the laboratory by growing first feeding fish together in the same system with live saltwater rotifers. This "polyculture" strategy promotes high growth and survival with minimal labor and disturbance to the larvae.

Education

JoVE Core - Biology

Osmoregulation in Fishes

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2019

When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments? Fish employ osmoregulatory strategies to balance bodily levels of water and dissolved ions (i.e., solutes), such as sodium and chloride. Imagine two solutions separated by a membrane that is permeable to water.

Dissection of Larval Zebrafish Gonadal Tissue

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Cited by 6 •

2017

Here, we present a protocol for isolating gonadal tissue of larval zebrafish, which will facilitate investigations of zebrafish sex differentiation and maintenance.

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