Mass Spectrometer Analysis
Mass Spectrometer Analysis - Related Videos
01:16
Mass Spectrometers
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2024
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions: Conversion of the gas-phase analyte atoms/molecules into a beam of positive or negative charged ions by ionization. Separation of the charged species based on their mass-to-charge ratio. Recording the relative abundance of each type of ion. In the ionization chamber of...
13:31
High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
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Cited by 42 •
2015
Here we present a protocol to build a rapid Brillouin spectrometer. Cascading virtually imaged phase array (VIPA) etalons achieve a measurement speed more than 1,000 times faster than traditional scanning Fabry-Perot spectrometers. This improvement provides the means for Brillouin analysis of tissue and biomaterials at low power levels in vivo.
11:18
A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer (SMPS-ICPMS)
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Cited by 9 •
2017
In this work a practical guide is provided, describing the different steps to establish the coupling of SMPS and ICPMS systems, and how to use them. Three descriptive examples are presented.
13:09
Mass Spectrometric Analysis of Glycosphingolipid Antigens
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Cited by 9 •
2013
A specific and sensitive method to gain insight into the expression profile of glycosphingolipid antigens in immune organs and cells is described. The method takes advantage of the ion trap mass spectrometry allowing step-wise fragmentation of glycosphingolipid molecules for structural analysis in comparison to chemically synthesized standards.
01:25
IR Spectrometers
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2024
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
FAQs
After ionization, electric or magnetic fields guide the resulting ions through the instrument and enable separation by mass-to-charge ratio. Ions with different ratios are distinguished as they pass through these fields, creating signals that the detector can measure. This mechanism converts chemical differences in the introduced sample into a spectrum suitable for interpretation.
Ion abundance supplies the intensity dimension of a mass spectrum. After the instrument separates ions by mass-to-charge ratio, the detector records how much signal is associated with each separated ion. Engineers use that pattern alongside molecular composition and isotopic information to compare samples and identify notable composition changes or trace contaminants.
Isotopic patterns add a compositional clue beyond the presence of individual signals. Their arrangement in a mass spectrum can help engineers assess molecular composition and recognize differences among materials or samples. This is especially useful when bulk measurements appear similar, because the spectrum can expose composition changes or trace contaminants relevant to material evaluation and quality control.
Once a sample enters the instrument, it is ionized so that the resulting ions can be guided through electric or magnetic fields. The detector then records ion abundance and produces a mass spectrum. Interpreting that spectrum links measured signals to molecular composition, isotopic patterns, or contaminants, giving the analysis value for engineering investigations.
In engineering quality control, the analysis provides composition-focused evidence for checking whether fuels, polymers, pharmaceuticals, or advanced materials have changed. During failure analysis, engineers can examine spectra for composition differences associated with a problematic sample. The same evidence supports process monitoring by showing changes that may not be visible through bulk measurements.
Mass Spectrometer Analysis is useful when bulk measurements do not show an obvious difference because the spectrum can expose composition changes or trace contaminants. In engineering, that added chemical detail supports process monitoring, environmental testing, and evaluation of fuels, polymers, pharmaceuticals, and advanced materials, where composition forms part of the engineering assessment.