Cd45 Test
Cd45 Test - Related Videos
16:55
Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
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Cited by 13 •
2011
In this article we demonstrate the isolation of murine resident lung mesenchymal stem cells (lung MSC), their expansion, characterization and analysis of immunomodulatory properties.
08:47
Identifying Microglia and Peripheral Infiltrating Macrophages in the Injured Spinal Cords Using Flow Cytometry
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Cited by 1 •
2025
Identifying microglia (MG) and peripheral infiltrating macrophages (Mø) in injured spinal cords is difficult. In this protocol, flow cytometry (FCM) was used to identify M1-like MG, M2-like MG, M1-like Mø, and M2-like Mø, respectively. This technology can also be applied to other central nervous system diseases to understand the role of MG and Mø.
09:55
Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses
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Cited by 3 •
2022
This study uses flow cytometry and two different gating strategies on isolated perfused mice brain choroid plexuses; this protocol identifies the main immune cell subsets that populate this brain structure.
07:58
Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools
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Cited by 10 •
2020
Here, we present a protocol to use a curvelet transform-based, open-source MATLAB software tool for quantifying fibrillar collagen organization in the extracellular matrix of both normal and diseased tissues. This tool can be applied to images with collagen fibers or other types of line-like structures.
09:30
Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
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Cited by 2 •
2023
The protocol summarizes the best practices to minimize microbial bioburden in a cleanroom environment and includes strategies such as environmental monitoring, process monitoring, and product sterility testing. It is relevant for manufacturing and testing facilities that are required to meet current good tissue practice standards and current good manufacturing practice standards.
FAQs
Fluorescently labeled antibodies provide the CD45-specific signal, while light-scattering measurements add information about each particle’s physical characteristics. Flow cytometry combines these signals so investigators can identify leukocyte populations and examine their fluorescence and scattering patterns together. This multi-signal approach supports more informative immune profiling than either signal alone.
CD45 detection supplies a marker for most nucleated hematopoietic cells, whereas nonhematopoietic material generally lacks that leukocyte-associated signal. In a mixed sample, comparing antibody fluorescence with light-scattering behavior helps separate white blood cells from other material. This distinction improves interpretation of cellular composition and supports assessment of sample quality.
Changes in the abundance or phenotype of CD45-detected leukocyte populations can provide evidence of altered immune composition. During infection or another immune response, investigators can use these shifts to characterize how white-cell populations change rather than relying only on total sample appearance. The resulting profile supports studies of host responses to pathogens.
A basic workflow begins with a sample containing cells, exposure to fluorescently labeled antibodies that bind CD45, and flow-cytometric measurement of fluorescence and light scattering. Investigators then use the combined signals to identify and characterize leukocyte populations. The procedure therefore links molecular recognition by the antibody with population-level cellular analysis.
In leukemia classification, CD45 testing contributes an immunological dimension to cell characterization. Because the assay detects a leukocyte-associated membrane protein and can be combined with light-scattering and fluorescence information, it helps investigators evaluate cellular populations within a sample. Its value lies in supporting classification alongside broader flow-cytometric characterization.
For sample quality assessment, the assay can indicate whether the analyzed material contains the expected leukocyte signal and how cellular populations are represented. In host-pathogen research, the same measurements allow investigators to examine changes in leukocyte abundance or phenotype during immune responses. Thus, one assay connects sample evaluation with infection-focused immune profiling.