Chimeric Tcr Construct
Chimeric Tcr Construct - Related Videos
11:21
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
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Cited by 3 •
2017
The current protocol combines single cell paired human TCR alpha and beta chain sequencing with streamlined generation of retroviral vectors compatible with in vitro and in vivo TCR expression.
19:05
Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
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Cited by 9 •
2015
This manuscript describes how to conduct (single molecule) Förster Resonance Energy Transfer (FRET)- based assays to measure the binding dynamics between T-cell antigen receptor (TCR) and antigenic peptide-loaded MHC molecules as they occur within the immunological synapse of a T-cell in contact with a functionalized planar supported lipid bilayer.
11:39
Identification of Functional Protein Regions Through Chimeric Protein Construction
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2019
Structurally related proteins frequently exert distinct biological functions. The exchange of equivalent regions of these proteins in order to create chimeric proteins constitutes an innovative approach to identify critical protein regions that are responsible for their functional divergence.
07:30
HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice
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Cited by 8 •
2016
This article describes how to inject viral vectors into the mouse frontal cortex to test behavioral assays that require GPCR heteromeric formation.
16:10
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
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Cited by 13 •
2012
The compartmentalization of proteins either within the plasma membrane or into intracellular locations is one regulatory mechanism that can greatly influence signaling outcomes; hence, to understand signaling it is important to study the spatial and temporal behavior of the proteins involved. We describe here a TIRF microscopy based system to study signal transduction in T cells, but is broadly applicable.
FAQs
The variable regions provide the recognition interface for a selected peptide when that peptide is presented by a compatible major histocompatibility complex, or MHC, molecule. This means antigen specificity depends not only on the peptide itself but also on its presentation context. In infection studies, that relationship helps investigators examine antigen-specific T-cell responses.
Associated signaling domains convert receptor engagement into intracellular activation signals. These signals can support T-cell proliferation and cytotoxic activity, linking molecular recognition to functional immune responses. Examining this connection allows researchers to assess whether a construct merely recognizes an antigen or also promotes the cellular behaviors needed for effective immune activity.
These design strategies create an engineered receptor with selected recognition and signaling features rather than relying unchanged on a native T-cell receptor. Combining components or modifying native regions can therefore support deliberate redirection toward a chosen antigen. The resulting construct provides a system for studying how receptor architecture relates to antigen-specific T-cell function.
MHC presentation is a key condition for recognition because the receptor variable regions bind the antigen as a peptide-MHC complex. A construct may therefore be evaluated in the context of antigen-presenting cells or infected cells that display the relevant peptide through MHC. This framework connects receptor specificity with the biology of antigen presentation.
Researchers can use these constructs to redirect T cells toward cells displaying selected pathogen-associated antigens. The approach supports investigation of host-pathogen interactions and antigen-specific immunity by linking a defined receptor target with measurable T-cell outcomes, including proliferation or cytotoxic activity. It is particularly relevant when studying immune responses to persistent infections.
A chimeric TCR construct can serve as a platform for evaluating engineered cellular therapies and for examining whether redirected T cells respond to selected antigens. Studies may focus on receptor-mediated activation, proliferation, or cytotoxic activity in relevant antigen-presenting settings. These observations can inform efforts to improve immune responses against persistent infections.