Interferon Gamma
Interferon Gamma - Related Videos
05:28
Assay for the Identification of Interferon-Gamma Secretion in Murine Lymphoid Organs
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2025
This video demonstrates an imaging method to visualize in situ interferon-gamma (IFN-γ) secretion in the mouse spleen. Following bacterial infection and the induction of IFN-γ accumulation inside T cells and natural killer cells, the spleen is harvested from the mouse. Upon obtaining tissue sections, immunostaining is performed to visualize IFN-γ inside the cells.
09:11
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
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Cited by 3 •
2019
Here, we describe a simple confocal imaging method to visualize the in situ localization of cells secreting the cytokine Interferon gamma in murine secondary lymphoid organs. This protocol can be extended for the visualization of other cytokines in diverse tissues.
08:13
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
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Cited by 3 •
2019
The development of the interferon-gamma ELISpot assay for guinea pig PBMCs allows the characterization of T-cell responses in this highly relevant model for studying infectious diseases. We have applied the assay to measure T cell responses associated with intradermal delivery of DNA vaccines.
10:10
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
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2016
This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen.
13:41
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
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Cited by 9 •
2012
Characterizing T-cell epitopes of pathogens that cause localized infections such as human papillomavirus is a challenge because of limited number of T cells in circulation. A method is described in which rare T cells were isolated and were characterized starting with a very small number of cells.
FAQs
Receptor binding activates the JAK-STAT signaling pathway, which changes gene expression inside the responding cell. Those transcriptional changes enhance macrophage activity, improve antigen presentation, and promote inflammatory signaling. This mechanism converts an extracellular immune signal into coordinated cellular responses that can strengthen host defense against pathogens residing within cells.
Natural killer cells and activated T lymphocytes are the main sources identified in this pathway. Their production links early cellular immune activity with responses that develop after lymphocyte activation. Because these cells can stimulate macrophage function and antigen presentation through interferon gamma signaling, they help connect pathogen recognition, cellular activation, and broader immune coordination.
The outcome depends on whether signaling remains appropriately regulated. Effective activity supports macrophage activation, antigen presentation, and inflammatory responses that help resist infection. Excessive or poorly controlled activity, however, can contribute to tissue damage and chronic inflammation. Studying this balance is therefore important when interpreting immune protection alongside possible pathological effects.
Researchers can measure the pathway to examine immune function or investigate responses to infection, while manipulating it can reveal how signaling affects cellular activity and inflammation. The overview supports these approaches as research tools rather than specifying a single assay or intervention. Results can help connect interferon gamma activity with macrophage responses, antigen presentation, and disease-related immune outcomes.
Its role is especially relevant to host resistance against intracellular pathogens, including bacteria, viruses, and parasites. Infection studies examine how signaling supports cellular immune responses that act against organisms located within host cells. This focus helps researchers investigate why immune activation may improve resistance in some infections while also requiring regulation to limit inflammatory tissue injury.
The pathway provides a way to study how immune signals coordinate cellular defenses and shape responses to infectious disease. In vaccine research, investigators can examine interferon gamma as part of immune-function analysis. In immunotherapy research, measuring or manipulating the pathway helps evaluate whether changing its activity could enhance protective responses or contribute to excessive inflammation.