Murine Stromal Cells
Murine Stromal Cells - Related Videos
08:40
Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract
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Cited by 44 •
2015
The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.
05:47
Isolation of Murine Lymph Node Stromal Cells
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Cited by 34 •
2014
Isolation of lymph node stromal cells is a multistep procedure including enzymatic digestion and mechanical disaggregation to obtain fibroblastic reticular cells, lymphatic and blood endothelial cells. In the described procedure, a short digestion is combined with automated mechanical disaggregation to minimize surface marker degradation of viable lymph node stromal cells.
03:58
Generating Schwann Cell-Like Cells from Bone Marrow Stromal Cells
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2025
This video demonstrates a method for obtaining Schwann cell-like cells from multipotent bone marrow stromal cells (BMSCs). Initially, the BMSCs undergo hypoxia treatment, followed by directed differentiation into neuronal progenitor cells. Subsequently, specific media and culture conditions are employed to induce the transformation of these progenitor cells into Schwann cell-like cells.
07:22
Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
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Cited by 151 •
2013
Primary white preadipocytes isolated from white adipose tissues in mice can be differentiated into beige/brite cells. Presented here is a reliable cellular model system to study the molecular regulation of "browning" of white fat.
08:37
Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
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Cited by 86 •
2017
This study presents a standardized and validated method for the isolation and culture of primary mouse endometrial stromal and epithelial cells, which can be used in a coculture system to study in vitro decidualization.
FAQs
Extracellular-matrix production provides a local structural framework, while cell adhesion organizes physical contacts among stromal, immune, epithelial, and vascular cells. Together, these features help determine how cells remain positioned and interact within a tissue-like environment. In experimental models, changing these interactions can clarify how local organization influences leukocyte behavior, inflammation, and tissue repair.
Cytokines and chemokines act as soluble signals released by stromal cells to regulate nearby immune activity. Their effects can influence leukocyte survival, migration, and activation, linking the local tissue environment to broader inflammatory responses. Measuring these signaling effects in co-culture systems helps investigators examine how stromal cells shape immune-cell behavior rather than studying immune cells in isolation.
Primary murine stromal cells and stromal cell lines provide complementary experimental systems for investigating tissue-support functions. Using both can support controlled comparisons of immune-cell interactions, inflammatory signaling, or pathogen effects on tissue niches. This distinction is useful when interpreting whether an observed response reflects the behavior of a particular cellular model or a broader stromal contribution.
A co-culture model places murine stromal cells together with relevant immune cells so investigators can examine communication within a controlled local environment. Epithelial or vascular cells may also be included when the experiment addresses tissue organization. Researchers can then compare cellular interactions and inflammatory responses under homeostatic or inflammatory conditions without relying only on isolated cell populations.
These models connect cellular mechanisms with changes occurring in a tissue-like setting. They can be used to examine immune-cell recruitment, inflammatory signaling, tissue repair, and interactions among immune, epithelial, vascular, and stromal populations. Because the system supports controlled experimental comparisons, investigators can relate specific cellular interactions to disease progression or responses to treatment.
In infection studies, stromal-cell systems help investigators examine how pathogens affect tissue niches and how those altered environments shape host responses. Co-cultures and tissue-mimicking models can also test inflammatory signaling and treatment responses in a controlled context. This approach adds tissue-level support information to analyses that might otherwise focus mainly on pathogen or immune-cell behavior.