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JoVE Journal
Immunology and Infection

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Isolation of Murine Lymph Node Stromal Cells
 

Isolation of Murine Lymph Node Stromal Cells

Article doi: 10.3791/51803
August 19th, 2014

Summary August 19th, 2014

Isolation of lymph node stromal cells is a multistep procedure including enzymatic digestion and mechanical disaggregation to obtain fibroblastic reticular cells, lymphatic and blood endothelial cells. In the described procedure, a short digestion is combined with automated mechanical disaggregation to minimize surface marker degradation of viable lymph node stromal cells.

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