Asc Sheet Transplantation
Asc Sheet Transplantation - Related Videos
08:06
Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model
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Cited by 32 •
2017
Adipose-derived stem cells (ASCs) are easily isolated and harvested from the fat of normal rats. ASC sheets can be created using cell-sheet engineering and can be transplanted into Zucker diabetic fatty rats exhibiting full-thickness skin defects with exposed bone and then covered with a bilayer of artificial skin.
05:57
ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection
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Cited by 14 •
2017
This study reports a successful method of endoscopical adipose tissue-derived stromal cell (ADSC)-sheet transplantation for esophageal stricture prevention after an extended endoscopic submucosal dissection (ESD) in a swine model.
07:48
Engineering a Bilayered Hydrogel to Control ASC Differentiation
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Cited by 12 •
2012
This protocol focuses on utilizing the inherent ability of stem cells to take cue from their surrounding extracellular matrix and be induced to differentiate into multiple phenotypes. This methods manuscript extends our description and characterization of a model utilizing a bilayered hydrogel, composed of PEG-fibrin and collagen, to simultaneously co-differentiate adipose-derived stem cells1.
08:15
Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology
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2026
This protocol presents a simplified, end-to-side suture anastomosis technique for murine cervical heart transplantation, providing a robust and reproducible model for studying transplant immunology.
09:24
Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
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Cited by 2 •
2014
For creation of highly organized structures of complex tissue, one must assemble multiple material and cell types into an integrated composite. This combinatorial design incorporates organ-specific layered cell sheets with two distinct biologically-derived materials containing a strong fibrous matrix base, and endothelial cells for enhancing new vessels formation.
FAQs
Preserving the sheet’s intact architecture keeps ASCs connected to neighboring cells and their surrounding matrix during transfer. Those cell-cell and cell-matrix interactions are not fully disrupted as they would be by complete dissociation. Retaining this organization may help the transplanted layer function as a coordinated regenerative interface at injured or diseased tissue.
The extracellular matrix gives the transplanted sheet a matrix-rich environment in addition to its cellular component. Because the sheet retains matrix components formed during culture, it may contribute directly to tissue remodeling and healing after placement. This feature distinguishes the approach from strategies focused only on delivering separated cells to a target site.
Transplanted ASC sheets can provide paracrine signals, meaning locally acting factors released by the cells that influence nearby tissue. These signals may support repair processes and complement the physical contribution of the cell layer and its matrix. Together, the signals and retained organization provide a basis for investigating ASC sheets in regenerative medicine.
Preparation begins by expanding adipose-derived stem cells under controlled culture conditions. The cells remain in culture until they form a contiguous layer rich in extracellular matrix. Researchers then harvest the sheet and place it on the target tissue without fully dissociating the cells, preserving important cell-cell and cell-matrix relationships during transplantation.
Medicine researchers are investigating this approach for wound repair, musculoskeletal regeneration, and other cell-based therapies. These applications reflect the goal of supporting healing and tissue remodeling at sites affected by injury or disease. The technique therefore connects cell-based treatment research with broader regenerative strategies rather than serving only one tissue type.
The intended outcomes include support for tissue repair, remodeling of the local tissue environment, and improved healing. These effects are linked to the sheet’s retained matrix components and the paracrine signals provided by its ASCs. The method is consequently studied as a way to deliver both organized cells and regenerative cues to a target site.