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JoVE Journal
Bioengineering

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Engineering a Bilayered Hydrogel to Control ASC Differentiation
 

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Article doi: 10.3791/3953
May 25th, 2012

Summary May 25th, 2012

This protocol focuses on utilizing the inherent ability of stem cells to take cue from their surrounding extracellular matrix and be induced to differentiate into multiple phenotypes. This methods manuscript extends our description and characterization of a model utilizing a bilayered hydrogel, composed of PEG-fibrin and collagen, to simultaneously co-differentiate adipose-derived stem cells1.

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