Digital Microscopy

Digital microscopy is the acquisition, processing, and analysis of microscope images in digital form, enabling specimens to be viewed and shared on electronic displays rather than solely through eyepieces. A camera or scanning system converts light from a magnified specimen into digital data, while software supports image enhancement, measurement, annotation, storage, and retrieval. In medicine, digital microscopy assists histopathology, cytology, hematology, and medical education by supporting tissue evaluation, documentation, and remote consultation. Quantitative image analysis can also help identify cellular features and standardize assessments, strengthening research workflows and contributing to the development of computer-assisted diagnostic methods.

Digital Microscopy - Related Videos

Research

JoVE Journal - Medicine

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

0 Views •

Cited by 18 •

2016

Accurate assessment of anti-inflammatory effects is of utmost importance for the evaluation of potential new drugs for the treatment of inflammatory bowel disease. Digital holographic microscopy provides assessment of inflammation in murine and human colonic tissue samples as well as automated multimodal evaluation of epithelial wound healing in vitro.

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

0 Views •

Cited by 9 •

2014

The three-dimensional locations of weakly-scattering objects can be uniquely identified using digital inline holographic microscopy (DIHM), which involves a minor modification to a standard microscope. Our software uses a simple imaging heuristic coupled with Rayleigh-Sommerfeld back-propagation to yield the three-dimensional position and geometry of a microscopic phase object.

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

0 Views •

Cited by 19 •

2017

Digital holographic microscopy (DHM) is a volumetric technique that allows imaging samples 50-100X thicker than brightfield microscopy at comparable resolution, with focusing performed post-processing. Here DHM is used for identifying, counting, and tracking microorganisms at very low densities and compared with optical density measurements, plate count, and direct count.

Visualizing Non-lytic Exocytosis of Cryptococcus neoformans from Macrophages Using Digital Light Microscopy

0 Views •

Cited by 7 •

2014

We describe how to visualize macrophage-C. neoformans (Cn) interactions in real time, with specific emphasis on the process of non-lytic exocytosis using digital light microscopy. Using this technique individually infected macrophages can be studied to ascertain various aspects of this phenomenon.

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

0 Views •

Cited by 12 •

2016

A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.

View All Results

FAQs

Related Topics