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JoVE Journal
Biology

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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
 

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Article DOI: 10.3791/50488
February 8th, 2014

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Summary February 8th, 2014

The three-dimensional locations of weakly-scattering objects can be uniquely identified using digital inline holographic microscopy (DIHM), which involves a minor modification to a standard microscope. Our software uses a simple imaging heuristic coupled with Rayleigh-Sommerfeld back-propagation to yield the three-dimensional position and geometry of a microscopic phase object.

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