Lung Pathogens
Lung Pathogens - Related Videos
10:39
Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens
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Cited by 22 •
2013
We describe a method for tracking the endomembrane rupture elicited by the intracellular bacteria Shigella flexneri and Mycobacterium tuberculosis upon host cell invasion. Our assay makes use of CCF4, a host cytoplasmic FRET probe in live or fixed cells. This reporter is degraded by an enzyme activity present on the bacterial surface.
07:06
A Simple Method of Mouse Lung Intubation
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Cited by 50 •
2013
This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.
01:47
Lung Capacity
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2019
The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures. There are four defined lung volume measures: tidal volume, inspiratory reserve volume, expiratory reserve volume, and residual volume. Tidal volume is the amount of air inhaled and exhaled in a normal breath. Inspiratory...
05:35
Lung Instillation and Fixation: Introducing Liquid into the Trachea and Fixing Lungs to Measure the Fixed Lung Volume
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2023
This video describes a simple and reliable method of introducing liquid into the trachea for lung instillation, fixation, and lung volume measurement.
08:58
Development of a Neonatal Piglet Acute Lung Injury Model Recreating the Early Environment of Preterm Infant Lungs
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2025
This protocol describes the development of a neonatal piglet model of acute lung injury that models early pathogenic events occurring in the preterm lung, including sub-adequate surfactant quantity, hyperoxia, high-pressure ventilation, and inflammation, to facilitate understanding of molecular triggers of bronchopulmonary dysplasia and enhance therapeutic translation.
FAQs
After inhaled droplets or particles reach the respiratory tract, the microorganism may adhere to airway or alveolar surfaces and multiply. Its persistence depends partly on whether it can evade host defenses. Continued microbial growth and the resulting inflammatory response can damage respiratory tissue, disrupt normal gas exchange, and contribute to diseases such as pneumonia.
Different lung pathogens can produce disease through distinct combinations of adherence, multiplication, immune evasion, and tissue injury. Identifying which mechanisms are involved helps researchers connect a microorganism with particular respiratory effects rather than treating all infections as biologically identical. This distinction supports more accurate diagnosis, targeted antimicrobial treatment, and development of therapies for difficult infections.
Inflammation is part of the host response to microorganisms, but it can also contribute to tissue injury when infection persists or pathogen growth continues. Damage to airway or alveolar tissue may interfere with gas exchange and impair breathing. Studying this balance helps explain why the clinical consequences of infection depend on both microbial activity and the host response.
Microbiological and molecular testing provides evidence about the microorganism responsible for a respiratory infection. These approaches support accurate diagnosis by examining the infectious cause through complementary laboratory methods. The resulting information can guide antimicrobial treatment and help distinguish infections that may require different management, which is especially important when drug-resistant or emerging infections are being considered.
Antimicrobial treatment is important when a lung infection requires therapy directed at its causative microorganism. Diagnostic information helps guide that choice rather than relying only on the presence of respiratory disease. The need is particularly significant for drug-resistant infections, where understanding pathogen-specific mechanisms can support improved therapies and help researchers address reduced treatment effectiveness.
Infection-control and vaccination strategies address respiratory disease before or during transmission and infection. Their development depends on understanding how pathogens enter the respiratory tract, interact with host defenses, and cause tissue injury. In medicine, these strategies complement diagnostic testing and treatment by helping limit spread, reduce preventable disease, and improve outcomes during respiratory infections.