Dendritic Protein Labeling
Dendritic Protein Labeling - Related Videos
07:34
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
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Cited by 28 •
2011
Dendritic cells uptake antigens and migrate towards immune organs to present processed antigens to T cells. Qdot nanocrystal labeling provides a long-lasting and stable fluorescent signal. This allows tracking of dendritic cells to different organs by fluorescent microscopy.
07:49
Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall
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Cited by 11 •
2011
To understand how complex cell shapes, such as neuronal dendrites, are achieved during development, it is important to be able to accurately assay microtubule organization. Here we describe a robust immunohistological labeling method to examine microtubule organization of dendritic arborization neuron sensory dendrites, trachea, muscle, and other Drosophila larva body wall tissues.
06:48
Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
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Cited by 3 •
2016
The on-bead method for labeling antibodies with small molecules enables labeling of a small amount of antibodies directly from cell media. This method is compatible with amine and thiol chemistry, and can handle multiple samples in parallel, manually or using automated platforms.
13:50
Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
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Cited by 13 •
2013
The localization and distribution of proteins provide important information for understanding their cellular functions. The superior spatial resolution of electron microscopy (EM) can be used to determine the subcellular localization of a given antigen following immunohistochemistry. For tissues of the central nervous system (CNS), preserving structural integrity while maintaining antigenicity has been especially difficult in EM studies. Here, we adopt a procedure that has been used to preserve...
11:56
Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
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Cited by 24 •
2014
We describe a method to label protein on the surface of living neurons using a specific polyclonal antibody to extracellular epitopes. Protein bound by the antibody on the cell surface and subsequently internalized via endocytosis can be distinguished from protein remaining on, or trafficked to, the surface during the incubation.
FAQs
The main mechanistic distinction is whether labeling preserves a snapshot or tracks behavior over time. Fixation halts the cellular state before antibody detection, allowing researchers to examine where a target resides at that point. Genetically encoded fluorescent tags remain associated with the protein in living neurons, making redistribution and trafficking observable.
Fixation and permeabilization control antibody-based detection in fixed neurons. Fixation prepares the cells for labeling, while permeabilization enables fluorescently conjugated antibodies to reach target epitopes inside them. The resulting fluorescent signal marks the recognized proteins, allowing researchers to assess their spatial pattern in dendrites and at synapses.
Genetically encoded fluorescent tags are most informative when the question concerns protein behavior in living neurons. The tags report protein distribution and trafficking, allowing researchers to examine where a protein is located and how it moves within dendrites. This approach is therefore suited to studying dynamic organization rather than only a single fixed state.
A fixed-cell workflow begins by fixing the neurons, followed by permeabilization so fluorescently conjugated antibodies can reach target epitopes inside them. After antibody binding, researchers examine the fluorescent pattern across dendrites and synaptic regions. This workflow supports spatial measurements, including protein location and abundance, rather than direct observation of movement in living cells.
Dendritic protein labeling can connect molecular measurements with neuronal architecture. Researchers can compare protein signal with dendritic spine structure and determine whether receptors, scaffolding proteins, or cytoskeletal components are concentrated at synapses. These paired observations help relate protein organization to synaptic sites and to changes associated with neuronal activity.
These methods support neuroscience studies that connect protein organization with neuronal changes. By examining receptors, scaffolding proteins, and cytoskeletal components in dendrites, researchers can investigate synaptic plasticity and neural development. The same measurements also provide a way to study protein organization associated with mechanisms disrupted in neurological disease, especially when synapses and dendritic structure are central.