Fpr-rs3-i-venus Mice
Fpr-rs3-i-venus Mice - Related Videos
10:11
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Cited by 1 •
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Here, we describe a physiological approach that allows identification and in-depth analysis of a defined population of sensory neurons in acute coronal tissue slices of the mouse vomeronasal organ using whole-cell patch-clamp recordings.
03:34
Establishing a Whole-Cell Patch Clamp for Electrophysiological Recording from Vomeronasal Sensory Neurons
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2025
The protocol video describes establishing a whole-cell patch clamp for electrophysiological recording from the sensory neurons of the vomeronasal organ (VNO) in mice. A coronal VNO slice with fluorescently labeled sensory neurons is placed in a recording setup. A labeled neuron is patched with a recording pipette in a whole-cell configuration for electrophysiological recording in response to chemical stimuli.
08:41
Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
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Cited by 6 •
2021
This protocol describes the dynamics of viral infections using luciferase- and fluorescence-expressing recombinant (r)SARS-CoV-2 and an in vivo imaging systems (IVIS) in K18 hACE2 transgenic mice to overcome the need of secondary approaches required to study SARS-CoV-2 infections in vivo.
09:43
Video-oculography in Mice
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Cited by 23 •
2012
Video-oculography is a very quantitative method to investigate ocular motor performance as well as motor learning. Here, we describe how to measure video-oculography in mice. Applying this technique on normal, pharmacologically-treated or genetically modified mice is a powerful research tool to explore the underlying physiology of motor behaviors.
07:56
Parabiosis in Mice: A Detailed Protocol
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Cited by 188 •
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Parabiotic joining of two organisms leads to the development of a shared circulatory system. In this protocol, we describe the surgical steps to form a parabiotic connection between a wild-type mouse and a constitutive GFP-expressing mouse.
FAQs
The Venus signal is produced when regulatory elements associated with Fpr-rs3 activate the reporter construct. Fluorescence therefore provides a visible readout of cells linked to Fpr-rs3 expression, rather than relying only on anatomical appearance. Researchers can use this signal to locate gene-defined populations in neural tissue and examine their organization within specific regions.
Patterns of Venus-positive cells can show where Fpr-rs3-expressing populations are concentrated, dispersed, or arranged across tissue sections. This spatial information helps researchers map the distribution and organization of a gene-defined population. Comparing these patterns across neural regions can connect Fpr-rs3 expression with particular anatomical structures and support focused studies of sensory-related circuitry.
A gene-defined population gives researchers a molecular reference for examining neural cells that may share a particular functional or circuit-related identity. In Fpr-rs3 studies, labeling permits gene expression to be considered alongside cell location, neural connections, sensory processing, and behavior. This combination can provide more specific biological context than studying neural tissue only by gross anatomy.
The same fluorescent labeling can be examined in tissue sections to determine where Fpr-rs3-associated cells occur, or used during neural circuitry studies to investigate their connections. These approaches answer complementary questions: tissue analysis emphasizes distribution and organization, whereas circuit analysis addresses how labeled populations relate to connected neural structures. Together, they connect cellular identity with network arrangement.
A study can begin by examining neural tissue from the mice for Venus fluorescence, identifying labeled cells in tissue sections, and documenting their distribution. Researchers can then analyze the labeled population in relation to neural circuitry, sensory processing, or behavior, depending on the study design. This workflow moves from cell identification to anatomical and functional interpretation.
These mice are suited to studies that need to locate and follow Fpr-rs3-expressing cells, including cell-type identification, circuit tracing, and mapping of neural populations. The model is particularly relevant when researchers want to examine olfactory functions or other sensory processes. Fluorescent labeling provides a consistent way to connect those questions with defined cellular populations.
By identifying cells associated with Fpr-rs3 expression, researchers can examine where those cells occur and how their connections fit within neural circuitry. They can then interpret that anatomical information alongside studies of sensory processing or behavior. In this way, the model supports investigation of whether a gene-defined population is relevant to olfactory or other neural functions.