Parabiosis in Mice: A Detailed Protocol

Journal

Medicine

JoVE Journal
Medicine

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JoVE Journal Medicine

Parabiosis in Mice: A Detailed Protocol

DOI:

10.3791/50556-v

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07:56 min

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October 06, 2013

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Paniz Kamran², Konstantina-Ioanna Sereti², Peng Zhao², Shah R. Ali, Irving L. Weissman, Reza Ardehali²

¹Department of Medicine-Division of Cardiology,University of California, Los Angeles, ²Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research,University of California, Los Angeles, ³Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine

Chapters

00:05Title
01:06Surgical Preparations
02:22Parabiosis Surgery
04:35Post-operative Recovery
05:23Confirmation and Reverse Procedure
07:05Anticipated Outcome Using GFP
07:36Conclusion

Summary

Automatic Translation

Parabiotic joining of two organisms leads to the development of a shared circulatory system. In this protocol, we describe the surgical steps to form a parabiotic connection between a wild-type mouse and a constitutive GFP-expressing mouse.

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Chapters

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