Head Cap Construction

Head cap construction is a neuroscience method for creating a stable, protective interface between implanted electrodes and the skull, enabling repeated access to neural signals in awake animals. The procedure positions recording or stimulation electrodes at targeted brain locations, secures them to the skull with anchoring materials, and integrates the leads into a compact cap that protects the implant and reduces movement-related strain. A well-constructed head cap supports chronic electrophysiological recordings during behavior, improves signal consistency across sessions, and allows researchers to relate neural activity to sensory processing, movement, learning, and other brain functions.

Head Cap Construction - Related Videos

Research

JoVE Journal - Biology

Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis

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Cited by 8 •

2010

Animal caps overexpressing gene product(s) are transplanted to the flank of developing Xenopus laevis embryos in order to establish whether tissue is determined.

Design and Construction of an Urban Runoff Research Facility

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Cited by 6 •

2014

This paper describes the design, construction, and function of a 1,000 m2 facility containing 24 individual 33.6 m2 field plots equipped for measuring total runoff volumes with time and collection of runoff subsamples at selected intervals for quantification of chemical constituents in the runoff water from simulated home lawns.

Research

JoVE Journal - Biology
Free Sample

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

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Cited by 13 •

2012

This method describes high yield in vitro synthesis of both capped and uncapped mRNA from a linearized plasmid containing the Gaussia luciferase (GLuc) gene. The RNA is purified and a fraction of the uncapped RNA is enzymatically capped using the Vaccinia virus capping enzyme. In the final step, the mRNA is transfected into HeLa cells and cell culture supernatants are assayed for luciferase activity.

Construction and Characterization of a Novel Vocal Fold Bioreactor

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Cited by 8 •

2014

A novel vocal fold bioreactor capable of delivering physiologically relevant, vibratory stimulation to cultured cells is constructed and characterized. This dynamic culture device, when combined with a fibrous poly(ε-caprolactone) scaffold, creates a vocal fold-mimetic environment that modulates the behaviors of mesenchymal stem cells.

Research

JoVE Journal - Biology
Free Sample

Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping

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Cited by 4 •

2011

The Ice-Cap method allows one to grow plants in 96-well plates and non-destructively harvest root tissue from each seedling. DNA extracted from this root tissue can be used for genotyping reactions. We have found that Ice-Cap works well for Arabidopsis thaliana, tomato, and rice seedlings.

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