Journal
/
Biology
/
Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping

Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping

DOI:
10.3791/3280-v

•

11:15 min

•

November 09, 2011

•

, , , ,
1Horticulture Department,University of Wisconsin-Madison, 2Department of Zoology,Oregon State University

Chapters

  • 00:05Title
  • 02:04Preparation of Ice-Cap Seedling Plates with Agar Growth Media
  • 02:53Sowing and Germination of Seedlings in Ice-Cap Seedling Plates
  • 03:52Transfer of Seedling Plates to the Ice-Cap Fountain
  • 05:46Harvesting of Root Tissue
  • 07:51DNA Extraction
  • 09:19Planting of Seedlings with the Desired Genotyping
  • 10:14Representative Results Illustrating Arabidopsis Seedlings Grown Using the Ice-Cap System
  • 10:36Conclusion

Summary

Automatic Translation

Automatic Translation

The Ice-Cap method allows one to grow plants in 96-well plates and non-destructively harvest root tissue from each seedling. DNA extracted from this root tissue can be used for genotyping reactions. We have found that Ice-Cap works well for Arabidopsis thaliana, tomato, and rice seedlings.

Tags

Ice-Cap MethodArabidopsisTomato Plants96-well PlatesHigh-throughput GenotypingBottleneckGenotyping PipelineHigh-throughput SolutionSeedlingsAgar Growth MediaDNA ExtractionRapid Freezing

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3280?article_type=v

Pyrosequencing: A Simple Method for Accurate Genotyping
check_url/3280?article_type=v

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
check_url/3280?article_type=v

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
check_url/3280?article_type=v

Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates
check_url/3280?article_type=v

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
check_url/3280?article_type=v

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
check_url/3280?article_type=v

Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates
check_url/3280?article_type=v

qPCRTag Analysis – A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
check_url/3280?article_type=v

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
check_url/3280?article_type=v

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

Read Article