Patient-specific Cells
Patient-specific Cells - Related Videos
12:09
Patient-specific Modeling of the Heart: Estimation of Ventricular Fiber Orientations
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Cited by 14 •
2013
A methodology to estimate ventricular fiber orientations from in vivo images of patient heart geometries for personalized modeling is described. Validation of the methodology performed using normal and failing canine hearts demonstrate that that there are no significant differences between estimated and acquired fiber orientations at a clinically observable level.
10:24
Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System
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Cited by 23 •
2015
The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.
01:58
Cell Specific Gene Expression
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2020
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
08:52
Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
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Cited by 49 •
2011
A rapid, simple and cost-effective protocol for the generation of donor-derived multivirus-specific CTLs (rCTL) for infusion to allogeneic hematopoietic stem cell transplant (HSCT) recipients at risk of developing CMV, Adv or EBV infections. This manufacturing process is GMP-compliant and should ensure the broader implementation of T-cell immunotherapy beyond specialized centers.
08:35
A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs
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Cited by 31 •
2017
Current ex vivo models of glioblastoma (GBM) are not optimized for physiologically relevant study of human tumor invasion. Here, we present a protocol for generation and maintenance of organotypic slice cultures from fresh human GBM tissue. A description of time-lapse microscopy and quantitative cell migration analysis techniques is provided.
FAQs
The key experimental advantage is preservation of the donor’s genetic background during reprogramming and neural differentiation. That background can carry biological variation associated with neurological health or disease, allowing investigators to examine cellular behavior in a patient-linked context rather than treating all samples as biologically interchangeable. This feature is especially relevant when disease mechanisms differ among individuals.
Reprogramming and differentiation serve different purposes. Reprogramming converts patient-derived somatic cells into induced pluripotent stem cells, while differentiation guides those cells toward neurons or other neural cell types. Separating these stages gives researchers a route from an individual’s cells to neural models that retain the donor’s genetic context, supporting targeted study of disease-associated cellular mechanisms.
Patient-specific cells can expose variation that a generalized model may not represent. Because each model retains the donor’s genetic background, researchers can compare disease-associated cellular mechanisms across individuals. This makes the approach relevant to precision medicine, where the goal is to develop treatments that are more predictive for particular patients rather than uniformly assuming that one treatment will have the same effects across all individuals.
A typical workflow begins with obtaining somatic cells from an individual, reprogramming them into induced pluripotent stem cells, and guiding differentiation into neurons or other neural cell types. The resulting cells are then used as laboratory models of the donor’s biology. This sequence links sample origin, cell-state conversion, and neural specialization in a single research framework.
Once neural cell types have been generated, investigators can use them to model neurodegenerative and neurodevelopmental diseases. The models help reveal disease-associated cellular mechanisms and provide a setting for drug screening. Their value lies in connecting observed cellular effects to a defined patient background, which can support evaluation of treatments intended to be more predictive.
In neuroscience, these cells are useful because neurological disorders may involve changes that become visible in relevant neural cell types. Differentiating patient-derived induced pluripotent stem cells into neurons or other neural cells creates a system for examining disease-linked features in the laboratory. The approach therefore connects human patient biology with experimental analysis of nervous-system disease.