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A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs
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JoVE Journal Medicine
A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs

A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs

DOI:
10.3791/53557-v

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08:35 min

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July 20, 2017

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, , ,
1Department of Neurosurgery,Stanford University Hospital, Stanford University School of Medicine, 2Inova Neuroscience Institute

Chapters

  • 00:05Title
  • 00:43Slice Culture Acquisition and Maintenance
  • 03:41GFP-expressing Retrovirus Glioblastoma (GBM) Cell Labeling and Time-lapse Single Photon Laser Scanning Confocal Imaging
  • 05:02Imaging Post-processing and Tumor Cell Tracking
  • 06:53Results: Representative Human GBM Imaging and Analyses
  • 08:15Conclusion

Summary

Automatic Translation

Automatic Translation

Current ex vivo models of glioblastoma (GBM) are not optimized for physiologically relevant study of human tumor invasion. Here, we present a protocol for generation and maintenance of organotypic slice cultures from fresh human GBM tissue. A description of time-lapse microscopy and quantitative cell migration analysis techniques is provided.

Tags

Keywords: GlioblastomaOrganotypic Slice CultureTumor Cell MigrationAnti-invasive DrugsNeurooncologyPrimary CellsCellular HeterogeneityAgarose EmbeddingVibratome Sectioning

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