Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Medicine

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

JoVE Journal Medicine
A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs
 

A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs

Article doi: 10.3791/53557
July 20th, 2017 •
1, 2, 2, 2
1Department of Neurosurgery, Stanford University Hospital, Stanford University School of Medicine, 2Inova Neuroscience Institute

Summary
Automatic Translation
July 20th, 2017

Current ex vivo models of glioblastoma (GBM) are not optimized for physiologically relevant study of human tumor invasion. Here, we present a protocol for generation and maintenance of organotypic slice cultures from fresh human GBM tissue. A description of time-lapse microscopy and quantitative cell migration analysis techniques is provided.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter