Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

Pappanaicken Kumaresan; Mathew Figliola; Judy S. Moyes; M. Helen Huls; Priti Tewari; Elizabeth J. Shpall; Richard Champlin; Laurence J.N. Cooper

doi:10.3791/52808

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Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

12,312 Views

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10:24 min

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October 05, 2015

DOI:

10.3791/52808-v

DOI:

10.3791/52808-v

•

10:24 min

October 05, 2015

•

8 Views

Pappanaicken Kumaresan*¹, Mathew Figliola*¹, Judy S. Moyes¹, M. Helen Huls¹, Priti Tewari¹, Elizabeth J. Shpall², Richard Champlin², Laurence J.N. Cooper¹

¹Division of Pediatrics,U.T. MD Anderson Cancer Center, ²Stem Cell Transplantation and Cellular Therapy,U.T. MD Anderson Cancer Center

Summary

The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.