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Immunology and Infection

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JoVE Journal Immunology and Infection
Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System
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Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

Article DOI: 10.3791/52808 • 10:24 min
October 5th, 2015 •
*1, *1, 1, 1, 1, 2, 2, 1
1Division of Pediatrics, U.T. MD Anderson Cancer Center, 2Stem Cell Transplantation and Cellular Therapy, U.T. MD Anderson Cancer Center
* These authors contributed equally

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Summary
Automatic Translation
October 5th, 2015

The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.

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