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Immunology and Infection
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Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System
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Immunology and Infection
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JoVE Journal Immunology and Infection
Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

DOI:
10.3791/52808-v

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10:24 min

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October 05, 2015

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, , , , , , ,
1Division of Pediatrics,U.T. MD Anderson Cancer Center, 2Stem Cell Transplantation and Cellular Therapy,U.T. MD Anderson Cancer Center

Chapters

  • 00:05Title
  • 02:00Automated Cell Enrichment
  • 05:03Cell Count Determination
  • 06:35Examination of the Separation Performance
  • 07:54Results: Enrichment of CMV-specific T Cells Using the CCS System
  • 09:29Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to manufacture pathogen-specific clinical-grade T cells using a bench-top, automated, second generation cell enrichment device that incorporates a closed cytokine capture system and does not require dedicated staff or use of a GMP facility. The cytomegalovirus pp65-specific-T cells generated can be directly administered to patients.

Tags

Automated Cell EnrichmentCytomegalovirus-specific T CellsClinical ApplicationsCytokine-capture SystemAllogeneic Hematopoietic Stem-Cell TransplantationViral-specific T CellsGamma-interferonCytokine Capture System (CCS)CliniMACS ProdigyMagnetic Activated Cell SortingCMV Pp65-specific T CellsAutomated Cell Enrichment DeviceGMP FacilitiesBlood Banking Facilities

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