Name: Bereiding van in agarkraal ingebedde Mycobacterium abscessus om immunocompetente muizen intratracheaal te inoculeren (Video) | JoVE Journal
Uploaded: 2025-04-25T13:20:00.000+00:00
Duration: 5 min 40 s
Description: Leer een protocol voor agarkraal-embedding van Mycobacterium abscessus voor intratracheale inoculatie bij immunocompetente muizen. Deze methode ondersteunt preklinisch antibioticumonderzoek.

ai_agent_IP

ai_agent

maintenance

instantTrialDurationMinutes

isBiopharma

enableNewMyJoveSectionDesign

educationFeatures

seoMetaApi

ai_copilot_institution_blacklist

ai_chat_institution_blacklist

freeAccessWindow

ssoRoleCapture

scheduledMaintenanceBanner

joveLabsFeatures

disable_autoplay_for_bots

extended_trial_duration

5 minutes

<ol><li>Dartois, V., Dick, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Drug+development+challenges+in+nontuberculous+mycobacterial+lung+disease%3A+Tb+to+the+rescue%5BTitle%5D)+AND+%22J+Exp+Med%22%5BJournal%5D)">Drug development challenges in nontuberculous mycobacterial lung disease: Tb to the rescue.</a> J Exp Med. 219 (6), e20220445(2022).</li>
<li>Martiniano, S. L., Nick, J. A., Daley, C. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Nontuberculous+mycobacterial+infections+in+cystic+fibrosis%5BTitle%5D)+AND+%22Clin+Chest+Med%22%5BJournal%5D)">Nontuberculous mycobacterial infections in cystic fibrosis.</a> Clin Chest Med. 43 (4), 697-716 (2022).</li>
<li>Maurer, F. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lack+of+antimicrobial+bactericidal+activity+in+mycobacterium+abscessus%5BTitle%5D)+AND+%22Antimicrob+Agents+Chemother%22%5BJournal%5D)">Lack of antimicrobial bactericidal activity in mycobacterium abscessus.</a> Antimicrob Agents Chemother. 58 (7), 3828-3836 (2014).</li>
<li>Wu, M. L., Aziz, D. B., Dartois, V., Dick, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ntm+drug+discovery%3A+Status%2C+gaps+and+the+way+forward%5BTitle%5D)+AND+%22Drug+Discov+Today%22%5BJournal%5D)">Ntm drug discovery: Status, gaps and the way forward.</a> Drug Discov Today. 23 (8), 1502-1519 (2018).</li>
<li>Egorova, A., Jackson, M., Gavrilyuk, V., Makarov, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pipeline+of+anti-mycobacterium+abscessus+small+molecules%3A+Repurposable+drugs+and+promising+novel+chemical+entities%5BTitle%5D)+AND+%22Med+Res+Rev%22%5BJournal%5D)">Pipeline of anti-mycobacterium abscessus small molecules: Repurposable drugs and promising novel chemical entities.</a> Med Res Rev. 41 (4), 2350-2387 (2021).</li>
<li>Nuermberger, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Using+animal+models+to+develop+new+treatments+for+tuberculosis%5BTitle%5D)+AND+%22Semin+Respir+Crit+Care+Med%22%5BJournal%5D)">Using animal models to develop new treatments for tuberculosis.</a> Semin Respir Crit Care Med. 29 (5), 542-551 (2008).</li>
<li>Nuermberger, E. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Preclinical+efficacy+testing+of+new+drug+candidates%5BTitle%5D)+AND+%22Microbiol+Spectr%22%5BJournal%5D)">Preclinical efficacy testing of new drug candidates.</a> Microbiol Spectr. 5 (3), (2017).</li>
<li>Nicola, F., Cirillo, D. M., Lore, N. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Preclinical+murine+models+to+study+lung+infection+with+mycobacterium+abscessus+complex%5BTitle%5D)+AND+%22Tuberculosis+%28Edinb%29%22%5BJournal%5D)">Preclinical murine models to study lung infection with mycobacterium abscessus complex.</a> Tuberculosis (Edinb). 138, 102301(2023).</li>
<li>Dartois, V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Preclinical+murine+models+for+the+testing+of+antimicrobials+against+mycobacterium+abscessus+pulmonary+infections%3A+Current+practices+and+recommendations%5BTitle%5D)+AND+%22Tuberculosis+%28Edinb%29%22%5BJournal%5D)">Preclinical murine models for the testing of antimicrobials against mycobacterium abscessus pulmonary infections: Current practices and recommendations.</a> Tuberculosis (Edinb). 147, 102503(2024).</li>
<li>Obregon-Henao, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Susceptibility+of+mycobacterium+abscessus+to+antimycobacterial+drugs+in+preclinical+models%5BTitle%5D)+AND+%22Antimicrob+Agents+Chemother%22%5BJournal%5D)">Susceptibility of mycobacterium abscessus to antimycobacterial drugs in preclinical models.</a> Antimicrob Agents Chemother. 59 (11), 6904-6912 (2015).</li>
<li>Lerat, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((In+vivo+evaluation+of+antibiotic+activity+against+mycobacterium+abscessus%5BTitle%5D)+AND+%22J+Infect+Dis%22%5BJournal%5D)">In vivo evaluation of antibiotic activity against mycobacterium abscessus.</a> J Infect Dis. 209 (6), 905-912 (2014).</li>
<li>Saliu, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Chronic+infection+by+nontypeable+haemophilus+influenzae+fuels+airway+inflammation%5BTitle%5D)+AND+%22ERJ+Open+Res%22%5BJournal%5D)">Chronic infection by nontypeable haemophilus influenzae fuels airway inflammation.</a> ERJ Open Res. 7 (1), (2021).</li>
<li>Rodgers, A. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Biologically+relevant+murine+models+of+chronic+pseudomonas+aeruginosa+respiratory+infection%5BTitle%5D)+AND+%22Pathogens%22%5BJournal%5D)">Biologically relevant murine models of chronic pseudomonas aeruginosa respiratory infection.</a> Pathogens. 12 (8), 1053(2023).</li>
<li>Hoover, J. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+robust+pneumonia+model+in+immunocompetent+rodents+to+evaluate+antibacterial+efficacy+against+s.+Pneumoniae%2C+h.+Influenzae%2C+k.+Pneumoniae%2C+p.+Aeruginosa+or+a.+Baumannii%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">A robust pneumonia model in immunocompetent rodents to evaluate antibacterial efficacy against s. Pneumoniae, h. Influenzae, k. Pneumoniae, p. Aeruginosa or a. Baumannii.</a> J Vis Exp. (119), e55068(2017).</li>
<li>Lore, N. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+aminoglycoside-modifying+enzyme+eis2+represents+a+new+potential+in+vivo+target+for+reducing+antimicrobial+drug+resistance+in+mycobacterium+abscessus+complex%5BTitle%5D)+AND+%22Eur+Respir+J%22%5BJournal%5D)">The aminoglycoside-modifying enzyme eis2 represents a new potential in vivo target for reducing antimicrobial drug resistance in mycobacterium abscessus complex.</a> Eur Respir J. 60 (6), 2201541(2022).</li>
<li>Riva, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+new+model+of+chronic+mycobacterium+abscessus+lung+infection+in+immunocompetent+mice%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">A new model of chronic mycobacterium abscessus lung infection in immunocompetent mice.</a> Int J Mol Sci. 21 (18), 6590(2020).</li>
<li>Chang, V., Phillips, P. P. J., Imperial, M. Z., Nahid, P., Savic, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+comparison+of+clinical+development+pathways+to+advance+tuberculosis+regimen+development%5BTitle%5D)+AND+%22BMC+Infect+Dis%22%5BJournal%5D)">A comparison of clinical development pathways to advance tuberculosis regimen development.</a> BMC Infect Dis. 22 (1), 920(2022).</li>
<li>Yang, S. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pathological+granuloma+fibrosis+induced+by+agar-embedded+mycobacterium+abscessus+in+c57bl%2F6jnarl+mice%5BTitle%5D)+AND+%22Front+Immunol%22%5BJournal%5D)">Pathological granuloma fibrosis induced by agar-embedded mycobacterium abscessus in c57bl/6jnarl mice.</a> Front Immunol. 14, 1277745(2023).</li>
<li>Poerio, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Combined+host-+and+pathogen-directed+therapy+for+the+control+of+mycobacterium+abscessus+infection%5BTitle%5D)+AND+%22Microbiol+Spectr%22%5BJournal%5D)">Combined host- and pathogen-directed therapy for the control of mycobacterium abscessus infection.</a> Microbiol Spectr. 10 (1), e0254621(2022).</li>
<li>Pearce, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Inhaled+tigecycline+is+effective+against+mycobacterium+abscessus+in+vitro+and+in+vivo%5BTitle%5D)+AND+%22J+Antimicrob+Chemother%22%5BJournal%5D)">Inhaled tigecycline is effective against mycobacterium abscessus in vitro and in vivo.</a> J Antimicrob Chemother. 75 (7), 1889-1894 (2020).</li>
<li>De Groote, M. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Gm-csf+knockout+mice+for+preclinical+testing+of+agents+with+antimicrobial+activity+against+mycobacterium+abscessus%5BTitle%5D)+AND+%22J+Antimicrob+Chemother%22%5BJournal%5D)">Gm-csf knockout mice for preclinical testing of agents with antimicrobial activity against mycobacterium abscessus.</a> J Antimicrob Chemother. 69 (4), 1057-1064 (2014).</li>
<li>Facchini, M., De Fino, I., Riva, C., Bragonzi, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Long+term+chronic+pseudomonas+aeruginosa+airway+infection+in+mice%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Long term chronic pseudomonas aeruginosa airway infection in mice.</a> J Vis Exp. (85), e51019(2014).</li>
<li>Degiacomi, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+novel+drug+candidate+vomg+kills+mycobacterium+abscessus+and+other+pathogens+by+inhibiting+cell+division%5BTitle%5D)+AND+%22Int+J+Antimicrob+Agents%22%5BJournal%5D)">The novel drug candidate vomg kills mycobacterium abscessus and other pathogens by inhibiting cell division.</a> Int J Antimicrob Agents. 64 (4), 107278(2024).</li>
<li>Rimal, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((T405%2C+a+new+penem%2C+exhibits+in+vivo+efficacy+against+m.+Abscessus+and+synergy+with+beta-lactams+imipenem+and+cefditoren%5BTitle%5D)+AND+%22Antimicrob+Agents+Chemother%22%5BJournal%5D)">T405, a new penem, exhibits in vivo efficacy against m. Abscessus and synergy with beta-lactams imipenem and cefditoren.</a> Antimicrob Agents Chemother. 66 (6), e0053622(2022).</li>
<li>Maggioncalda, E. C., Story-Roller, E., Ammerman, N. C., Nuermberger, E. L., Lamichhane, G. Progressive mycobacterium abscessus lung infection in c3heb/fej mice associated with corticosteroid administration. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aMaggioncalda%2C+EC+%22bioRxiv%22">bioRxiv</a>. , (2018).</li>
</ol>

<table><tbody><tr><td>125-mL fles</td><td>Corning</td><td>8388</td><td>Sterieel</td></tr><tr><td>3 x 3 buisjeshouder</td><td>Fisher</td><td>5972-0030PK</td><td>Gedesinfecteerd voor gebruik</td></tr><tr><td>3D geprinte muisstandaard</td><td>Transworld Marketing&nbsp;</td><td>op maat ontworpen en geproduceerd</td><td>Gedesinfecteerd voor gebruik</td></tr><tr><td>48-wel plate voor verdunning</td><td>Celltreat&nbsp;</td><td>229192</td><td>Sterieel</td></tr><tr><td>50-mL centrifugeerbuizen</td><td>Greiner Bio-One</td><td>227261</td><td>Sterieel</td></tr><tr><td>Anesethia-machine</td><td>E-Z Systems</td><td>EZ-AF9000 SYSTEM</td><td>n/a</td></tr><tr><td>Avanti J-15R&nbsp;</td><td>Beckman Coulter</td><td>&nbsp;B99517</td><td>Centrifuge</td></tr><tr><td>Barrier Pipette Tips in Lift-off Lid Rack 1000G</td><td>Thermo Scientific ART&nbsp;</td><td>21-236-2A</td><td>Sterieel</td></tr><tr><td>Biohazard-zak</td><td>Fisherbrand</td><td>22-044561</td><td></td></tr><tr><td>Connectorring&nbsp;</td><td>pluriSelect</td><td>41-50000-03</td><td></td></tr><tr><td>Wattenstaafjes met katoenen punt</td><td>Puritan&nbsp;</td><td>22-029-571</td><td>Sterieel</td></tr><tr><td>Beschikbare inoculatieschrapers Geel Sterieel 100 in ritssluitingszak</td><td>Cole-Parmer Essentials&nbsp;</td><td>03-391-562</td><td>Sterieel</td></tr><tr><td>Beschikbare poly-gelamineerde doek</td><td>Dynarex</td><td>19-310-671</td><td>Sterieel</td></tr><tr><td>Dulbecco's Phosphate-Buffered Saline</td><td>Thomas Scientific</td><td>21-031-CM</td><td>Sterieel</td></tr><tr><td>Trechter</td><td>pluriSelect</td><td>42-50000</td><td>Sterieel</td></tr><tr><td>GE Ultrospec 10</td><td>Sigma-Aldrich</td><td>GE80211630</td><td>Spectrofotometer</td></tr><tr><td>Gentlemacs Tissue Dissociator</td><td>Miltenyi Biotec</td><td>130-096-427</td><td>Niet hitte geactiveerd</td></tr><tr><td>Hamilton-spuit&nbsp;</td><td>Hamilton&nbsp;</td><td>80801</td><td>Gedesinfecteerd voor gebruik</td></tr><tr><td>Zware minerale olie</td><td>Sigma-Aldrich</td><td>330760-1L</td><td>Sterieel</td></tr><tr><td>Isofluraanoplossing 250 ml fles</td><td>Covetrus</td><td>29405</td><td>Huidcorrosie/-irritatie: categorie 2, Ernstig oogletsel/oogirritatie: categorie 2A, Specifiek toxische werking voor bepaalde organen (éénmalige blootstelling): categorie 3 Kan slaperigheid en duizeligheid veroorzaken, veroorzaakt ernstige oogirritatie, veroorzaakt huidirritatie. Niet inhaleren, gebruik in goed geventileerde ruimte.</td></tr><tr><td>M-buizen (gentleMACS)</td><td>Miltenyi Biotec</td><td>130-096-335</td><td>Sterieel</td></tr><tr><td>Magneetschudden MR Hei-Connect&nbsp;</td><td>Heidolph</td><td>505-40000-13-1</td><td></td></tr><tr><td>Magneetschudbeugel</td><td>Thomas Scientific</td><td>&nbsp;1181L08</td><td>Sterieel</td></tr><tr><td>Mechaans Pipet 100&ndash;1000 &micro;L&nbsp;</td><td>Gilson PIPETMAN L&nbsp;</td><td>FA10006M</td><td>Gedesinfecteerd voor gebruik</td></tr><tr><td>Mechaans Pipet 20&ndash;200 &micro;L&nbsp;</td><td>Gilson PIPETMAN L&nbsp;</td><td>FA10003MG</td><td>Gedesinfecteerd voor gebruik</td></tr><tr><td>Metaal Animal Feeding Needle 24 G</td><td>Braintree Scientific</td><td>N-VP 24G-1S</td><td>Moet worden gesteriliseerd (geautoclaveerd)</td></tr><tr><td>Middlebrook 7H10 Agar</td><td>Sigma-Aldrich</td><td>M0303-500G</td><td>Gesteriliseerd (geautoclaveerd)</td></tr><tr><td>Middlebrook 7H11 Agar</td><td>Thermo Fisher</td><td>R4554002</td><td>Gesteriliseerd (geautoclaveerd)</td></tr><tr><td>Middlebrook 7H9 medium</td><td>Beckton Dickinson</td><td>271310</td><td>Gesteriliseerd (geautoclaveerd)</td></tr><tr><td>Muis standaard onderdelen</td><td>TriMech Solutions&nbsp;</td><td>SRV-AMS-FDM</td><td>Gedesinfecteerd voor gebruik</td></tr><tr><td>Niet absorberende zijde hechtdraad</td><td>Fisher Scientific</td><td>18020-50</td><td>n/a</td></tr><tr><td>OADC vloeibare verrijking voor gebruik met Middlebrook-media 500 ml</td><td>Thermo Scientific Remel&nbsp;</td><td>R450605</td><td>Sterieel</td></tr><tr><td>PBS (10x), pH 7.4 Sterieel gefilterd 500 mL</td><td>Gibco</td><td>70-011-044</td><td>Sterieel</td></tr><tr><td>Peroxiguard-doekjes</td><td>Peroxigard</td><td>29221</td><td>n/a</td></tr><tr><td>Petrischalen met helder deksel Rond 100mmx 15mm</td><td>Fisherbrand&nbsp;</td><td>FB0875712</td><td>Sterieel</td></tr><tr><td>PluriStrainer 200 mm</td><td>pluriSelect</td><td>43-50200-03</td><td>Sterieel; celzeef</td></tr><tr><td>Roller-flessen</td><td>Corning</td><td>430165</td><td>Sterieel</td></tr><tr><td>Serologische pipet</td><td>Corning</td><td>4489</td><td>Sterieel</td></tr><tr><td>Spectrofotometercuvettes 1.5 mL</td><td>Fisher Scientific</td><td>14955127</td><td>Gesteriliseerd (geautoclaveerd)</td></tr><tr><td>Spuitstoppen of klemmen (nylon)</td><td>Trimech&nbsp;</td><td>SRV-AMS-MJF</td><td>Gedesinfecteerd voor gebruik</td></tr><tr><td>Tryptische sojabouillon</td><td>Sigma-Aldrich</td><td>T8907-500G</td><td>Sterieel</td></tr><tr><td>Tween 80&nbsp;</td><td>Fisher</td><td>170793</td><td>Gefilterd gesteriliseerd&nbsp;</td></tr><tr><td>Wide Bore Filtered Pipette Tips Lift-off Lid Rack 200G&nbsp;</td><td>Thermo Scientific ART</td><td>21-236-1A</td><td>Sterieel</td></tr></tbody></table>

bereiding van in agarkraal ingebedde mycobacterium abscessus om immunocompetente muizen intratracheaal te inoculeren

Preparation of Agar Bead Embedded Mycobacterium abscessus to Inoculate Immunocompetent Mice Intratracheally

Bereiding van in agarkraal ingebedde Mycobacterium abscessus om immunocompetente muizen intratracheaal te inoculeren

Robust mouse models of chronic infection with Mycobacterium abscessus, an environmental pathogen that preferably infects the lungs, are badly needed. ...

preparation-agar-bead-embedded-mycobacterium-abscessus-to-inoculate

Research

JoVE Journal

Biology

Biologie

Source: Bernut, A., et al., Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos. J. Vis. Exp. (2015).This video demonstrates a protocol for isolating and quantifying Mycobacterium abscessus from an infected zebrafish embryo. The procedure involves embryo euthanasia, tissue lysis, and homogenization to release the bacteria, followed by centrifugation and resuspension in a surfactant buffer. Finally, the bacterial suspension is serially diluted, plated...

Isolation and Cultivation of Bacteria from Zebrafish Embryos to Assess Infection Load

This video demonstrates the induction of systemic inflammation in an anesthetized mouse. The injection of mycobacterial antigen-adjuvant depots triggers cytokine release and localized inflammation. Lymphatic vessels open, enabling immune cell entry into lymph nodes and activation of T cells, which release pro-inflammatory cytokines, leading to widespread inflammation.

Subcutaneous Injection of Bacterial Antigen to Induce Systemic Inflammation in a Murine Model

This video demonstrates a method to generate a mouse model of primed mycobacterial uveitis (PMU) for studying post-infectious uveitis. Upon induction of PMU by intravitreal injection of heat-killed Mycobacterium tuberculosis in a mouse previously immunized with the same immunogen, the progression of chronic inflammation in the eye is analyzed using imaging and histopathological...

A Murine Model of Primed Mycobacterial Uveitis to Study Post-Infectious Uveitis

We beschrijven de agar-kralen methode om hardnekkige langdurige chronische Pseudomonas aeruginosa luchtweginfectie te vestigen in het muismodel.

Long Term Chronische<em&gt; Pseudomonas aeruginosa</em&gt; Luchtweginfectie in Muizen

Optisch transparante zebravis embryo's worden veel gebruikt om in realtime de interacties tussen pathogene micro-organismen en de aangeboren immuuncellen te bestuderen en te visualiseren. Micro-injectie van Mycobacterium abscessus, gecombineerd met fluorescentiebeeldvorming, wordt gebruikt om essentiële pathogene kenmerken zoals koordvorming in zebravis embryo's te onderzoeken.

Ontcijferen en Imaging Pathogenese en Cording van<em&gt; Mycobacterium abscessus</em&gt; In zebravis embryo&#39;s

Er wordt een test bij muizen beschreven om celmigratie van de huid naar de lymfeklieren te volgen, die de karakterisering van dendritische cellen in de huid mogelijk maakt die naar de lymfeklier zijn gemobiliseerd na infectie van de voetkussen met Bacille Calmette-Guérin.

A-CFSE gebaseerde test om de migratie van de huid van muizen dendritische cellen Onderzoek naar drainerende lymfeklieren tijdens infectie met<em&gt; Mycobacterium bovis</em&gt; Bacille Calmette-Guérin

We beschrijven de optische beeldvorming van muizen besmet met Mycobacterium tuberculosis (M. tuberculosis) met behulp van verslaggever enzym fluorescentie (REF). Dit protocol vergemakkelijkt de gevoelige en specifieke detectie van M. tuberculosis in pre-klinische diermodellen voor pathogenese, therapeutics en vaccin onderzoek.

Mycobacterium tuberculosis in muizen met verslaggever enzym fluorescentie Imaging

Hier presenteren we twee protocollen om te studeren fagocytensysteem -Mycobacterium abscessus interacties: de screening van een transposon mutant bibliotheek voor bacteriële intracellulaire deficiëntie en de gehaltebepaling van bacteriële intracellulaire transcriptome van RNA sequencing. Beide benaderingen inzicht geven in de genomische voordelen en aanpassingen van de transcriptomic verbetering van intracellulaire bacteriën fitness.

Identificatie van merkers van de virulentie van Mycobacterium abscessus voor intracellulaire replicatie in fagocyten

In dit rapport presenteren we een protocol waarmee de onderzoeker een muismodel van intraoculaire uveïtis kan genereren. Meer algemeen aangeduid als experimentele auto-immune uveïtis (EAU), dit gevestigde model legt vele aspecten van de menselijke ziekte vast. Hierin zullen we beschrijven hoe de progressie van de ziekte kan worden geïnduceerd en gevolgd met behulp van verschillende uitlezingen.

Experimentele auto-immune uveïtis: een intraoculair inflammatoir muismodel

Dit protocol schetst de stappen voor het induceren van Primed Myco bacteriële Uveïtis (PMU) bij muizen. Deze methode schetst de stappen om betrouwbare en robuuste oogontsteking in het muismodelsysteem te helpen produceren. Met behulp van dit protocol genereerden we uveitische ogen en niet-ontstoken medeogen van afzonderlijke dieren voor verdere evaluatie met immunologische, transcriptomische en proteomische assays.

Geprimed mycobacteriële uveïtis (PMU) als model voor post-infectieuze uveïtis

Hier presenteren we een protocol voor het uitvoeren van een lage dosis intradermale inoculatie van Mycobacterium avium in een muizenoor om blootstellingsachtige omstandigheden weer te geven.

Intradermale inoculatie van Mycobacterium avium in het oor van de muis

Leer een protocol voor agarkraal-embedding van Mycobacterium abscessus voor intratracheale inoculatie bij immunocompetente muizen. Deze methode ondersteunt preklinisch antibioticumonderzoek.

Bereiding van in agarkraal ingebedde Mycobacterium abscessus om immunocompetente muizen intratracheaal te inoculeren

Meer video's verkennen