Name: Scheiding en differentiële karakterisering van microbiële extracellulaire blaasjes in de darm bij zoutgevoelige ratten onder zoutrijke dieetomstandigheden (Video) | JoVE Journal
Uploaded: 2025-06-06T15:45:00.000+00:00
Duration: 7 min 21 s
Description: Leer hoe je microbiële extracellulaire blaasjes isoleert uit de darm van zoutgevoelige ratten met dichtheidsgradiëntcentrifugatie. Deze methode karakteriseert grootte, morfologie en samenstelling voor hypertensieonderzoek.

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<ol><li>Thursby, E., Juge, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Introduction+to+the+human+gut+microbiota%5BTitle%5D)+AND+%22Biochem+J%22%5BJournal%5D)">Introduction to the human gut microbiota.</a> Biochem J. 474 (11), 1823-1836 (2017).</li>
<li>Aschtgen, M. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Rotation+of+Vibrio+fischeri+Flagella+Produces+Outer+Membrane+Vesicles+That+Induce+Host+Development%5BTitle%5D)+AND+%22J+Bacteriol%22%5BJournal%5D)">Rotation of Vibrio fischeri Flagella Produces Outer Membrane Vesicles That Induce Host Development.</a> J Bacteriol. 198 (16), 2156-2165 (2016).</li>
<li>Gurung, S., Perocheau, D., Touramanidou, L., Baruteau, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+exosome+journey%3A+from+biogenesis+to+uptake+and+intracellular+signalling%5BTitle%5D)+AND+%22Cell+Comm+Signal%22%5BJournal%5D)">The exosome journey: from biogenesis to uptake and intracellular signalling.</a> Cell Comm Signal. 19 (1), 47(2021).</li>
<li>Avila-Calderón, E. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Outer+Membrane+Vesicles+of+Gram-Negative+Bacteria%3A+An+Outlook+on+Biogenesis%5BTitle%5D)+AND+%22Front+Microbiol%22%5BJournal%5D)">Outer Membrane Vesicles of Gram-Negative Bacteria: An Outlook on Biogenesis.</a> Front Microbiol. 12, 557902(2021).</li>
<li>Melo-Marques, I., Cardoso, S. M., Empadinhas, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Bacterial+extracellular+vesicles+at+the+interface+of+gut+microbiota+and+immunity%5BTitle%5D)+AND+%22Gut+Microbes%22%5BJournal%5D)">Bacterial extracellular vesicles at the interface of gut microbiota and immunity.</a> Gut Microbes. 16 (1), 2396494(2024).</li>
<li>Kim, N. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effect+of+gut+microbiota-derived+metabolites+and+extracellular+vesicles+on+neurodegenerative+disease+in+a+gut-brain+axis+chip%5BTitle%5D)+AND+%22Nano+Converg%22%5BJournal%5D)">Effect of gut microbiota-derived metabolites and extracellular vesicles on neurodegenerative disease in a gut-brain axis chip.</a> Nano Converg. 11 (1), 7(2024).</li>
<li>Ahmadi Badi, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microbiota-Derived+Extracellular+Vesicles+as+New+Systemic+Regulators%5BTitle%5D)+AND+%22Front+Microbiol%22%5BJournal%5D)">Microbiota-Derived Extracellular Vesicles as New Systemic Regulators.</a> Front Microbiol. 8, 1610(2017).</li>
<li>Barathan, M., Ng, S. L., Lokanathan, Y., Ng, M. H., Law, J. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+Profound+Influence+of+Gut+Microbiome+and+Extracellular+Vesicles+on+Animal+Health+and+Disease%5BTitle%5D)+AND+%22Int+J+Mol+Sci%22%5BJournal%5D)">The Profound Influence of Gut Microbiome and Extracellular Vesicles on Animal Health and Disease.</a> Int J Mol Sci. 25 (7), 4024(2024).</li>
<li>Maukonen, J., Saarela, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Human+gut+microbiota%3A+does+diet+matter%5BTitle%5D)+AND+%22Proc+Nutri+Soc%22%5BJournal%5D)">Human gut microbiota: does diet matter.</a> Proc Nutri Soc. 74 (1), 23-36 (2015).</li>
<li>Bier, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+High+Salt+Diet+Modulates+the+Gut+Microbiota+and+Short+Chain+Fatty+Acids+Production+in+a+Salt-Sensitive+Hypertension+Rat+Model%5BTitle%5D)+AND+%22Nutrients%22%5BJournal%5D)">A High Salt Diet Modulates the Gut Microbiota and Short Chain Fatty Acids Production in a Salt-Sensitive Hypertension Rat Model.</a> Nutrients. 10 (9), 1154(2018).</li>
<li>Aghamohammad, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Anti-inflammatory+and+immunomodulatory+effects+of+Lactobacillus+spp.+as+a+preservative+and+therapeutic+agent+for+IBD+control%5BTitle%5D)+AND+%22Immun+Inflamm+Dis%22%5BJournal%5D)">Anti-inflammatory and immunomodulatory effects of Lactobacillus spp. as a preservative and therapeutic agent for IBD control.</a> Immun Inflamm Dis. 10 (6), e635(2022).</li>
<li>Dong, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+Effects+of+High-Salt+Gastric+Intake+on+the+Composition+of+the+Intestinal+Microbiota+in+Wistar+Rats%5BTitle%5D)+AND+%22Med+Sci+Monit%22%5BJournal%5D)">The Effects of High-Salt Gastric Intake on the Composition of the Intestinal Microbiota in Wistar Rats.</a> Med Sci Monit. 26, e922160(2020).</li>
<li>Yan, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intestinal+Flora+Modulates+Blood+Pressure+by+Regulating+the+Synthesis+of+Intestinal-Derived+Corticosterone+in+High+Salt-Induced+Hypertension%5BTitle%5D)+AND+%22Circ+Res%22%5BJournal%5D)">Intestinal Flora Modulates Blood Pressure by Regulating the Synthesis of Intestinal-Derived Corticosterone in High Salt-Induced Hypertension.</a> Circ Res. 126 (7), 839-853 (2020).</li>
<li>Wang, X., Lang, F., Liu, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((High-Salt+Diet+and+Intestinal+Microbiota%3A+Influence+on+Cardiovascular+Disease+and+Inflammatory+Bowel+Disease%5BTitle%5D)+AND+%22Biology%22%5BJournal%5D)">High-Salt Diet and Intestinal Microbiota: Influence on Cardiovascular Disease and Inflammatory Bowel Disease.</a> Biology. 13 (9), 674(2024).</li>
<li>Qi, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microbiome-metabolome+analysis+insight+into+the+effects+of+high-salt+diet+on+hemorheological+functions+in+SD+rats%5BTitle%5D)+AND+%22Front+Nutri%22%5BJournal%5D)">Microbiome-metabolome analysis insight into the effects of high-salt diet on hemorheological functions in SD rats.</a> Front Nutri. 11, 1408778(2024).</li>
<li>Wu, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Insights+into+the+unique+roles+of+extracellular+vesicles+for+gut+health+modulation%3A+Mechanisms%2C+challenges%2C+and+perspectives%5BTitle%5D)+AND+%22Curr+Res+Microb+Sci%22%5BJournal%5D)">Insights into the unique roles of extracellular vesicles for gut health modulation: Mechanisms, challenges, and perspectives.</a> Curr Res Microb Sci. 7, 100301(2024).</li>
<li>Zhang, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effects+of+bacterial+extracellular+vesicles+derived+from+oral+and+gastrointestinal+pathogens+on+systemic+diseases%5BTitle%5D)+AND+%22Microbiol+Res%22%5BJournal%5D)">Effects of bacterial extracellular vesicles derived from oral and gastrointestinal pathogens on systemic diseases.</a> Microbiol Res. 285, 127788(2024).</li>
<li>Wang, H. X., Wang, Y. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Gut+Microbiota-brain+Axis%5BTitle%5D)+AND+%22Chinese+Med+J%22%5BJournal%5D)">Gut Microbiota-brain Axis.</a> Chinese Med J. 129 (19), 2373-2380 (2016).</li>
<li>Huang, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((A+cross-tissue+transcriptome+association+study+identifies+key+genes+in+essential+hypertension%5BTitle%5D)+AND+%22Front+Genet%22%5BJournal%5D)">A cross-tissue transcriptome association study identifies key genes in essential hypertension.</a> Front Genet. 14, 1114174(2023).</li>
<li>Gao, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Microbial+DNA+Enrichment+Promotes+Adrenomedullary+Inflammation%2C+Catecholamine+Secretion%2C+and+Hypertension+in+Obese+Mice%5BTitle%5D)+AND+%22J+Am+Heart+Assoc%22%5BJournal%5D)">Microbial DNA Enrichment Promotes Adrenomedullary Inflammation, Catecholamine Secretion, and Hypertension in Obese Mice.</a> J Am Heart Assoc. 11 (4), e024561(2022).</li>
<li>Chen, P. <a target="_blank" href="http://scholar.google.com/scholar?hl=en&safe=off&q=author%3aChen%2C+P+%22Gut+Microbiota+and+Pathogenesis+of+Organ+Injury%22">Gut Microbiota and Pathogenesis of Organ Injury</a>. , Springer. Singapore. (2020).</li>
<li>Xie, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((hPSCs-derived+brain+organoids+for+disease+modeling%2C+toxicity+testing+and+drug+evaluation%5BTitle%5D)+AND+%22Exp+Neurol%22%5BJournal%5D)">hPSCs-derived brain organoids for disease modeling, toxicity testing and drug evaluation.</a> Exp Neurol. 385, 115110(2024).</li>
<li>Liu, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+underlying+mechanisms+of+DNA+methylation+in+high+salt+memory+in+hypertensive+vascular+disease%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">The underlying mechanisms of DNA methylation in high salt memory in hypertensive vascular disease.</a> Sci Rep. 14 (1), 925(2024).</li>
<li>Li, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Wheat+%CE%B2-glucan+reduces+obesity+and+hyperlipidemia+in+mice+with+high-fat+and+high-salt+diet+by+regulating+intestinal+flora%5BTitle%5D)+AND+%22Int+J+Biol+Macromol%22%5BJournal%5D)">Wheat β-glucan reduces obesity and hyperlipidemia in mice with high-fat and high-salt diet by regulating intestinal flora.</a> Int J Biol Macromol. 288, 138754(2024).</li>
<li>Kerem, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Small+intestinal+microbiota+composition+altered+in+obesity-T2DM+mice+with+high+salt+fed%5BTitle%5D)+AND+%22Sci+Rep%22%5BJournal%5D)">Small intestinal microbiota composition altered in obesity-T2DM mice with high salt fed.</a> Sci Rep. 13 (1), 8256(2023).</li>
<li>Díez-Sainz, E., Milagro, F. I., Riezu-Boj, J. I., Lorente-Cebrián, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Effects+of+gut+microbiota-derived+extracellular+vesicles+on+obesity+and+diabetes+and+their+potential+modulation+through+diet%5BTitle%5D)+AND+%22J+Physiol+Biochem%22%5BJournal%5D)">Effects of gut microbiota-derived extracellular vesicles on obesity and diabetes and their potential modulation through diet.</a> J Physiol Biochem. 78 (2), 485-499 (2022).</li>
<li>Wu, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Liuzijue+training+improves+hypertension+and+modulates+gut+microbiota+profile%5BTitle%5D)+AND+%22Front+Cardiovasc+Med%22%5BJournal%5D)">Liuzijue training improves hypertension and modulates gut microbiota profile.</a> Front Cardiovasc Med. 10, 1075084(2023).</li>
<li>Qi, L. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Salvia+miltiorrhiza+bunge+extract+improves+the+Th17%2FTreg+imbalance+and+modulates+gut+microbiota+of+hypertensive+rats+induced+by+high-salt+diet%5BTitle%5D)+AND+%22J+Funct+Foods%22%5BJournal%5D)">Salvia miltiorrhiza bunge extract improves the Th17/Treg imbalance and modulates gut microbiota of hypertensive rats induced by high-salt diet.</a> J Funct Foods. 117, 106211(2024).</li>
<li>Wilck, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Salt-responsive+gut+commensal+modulates+T%28H%2917+axis+and+disease%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">Salt-responsive gut commensal modulates T(H)17 axis and disease.</a> Nature. 551 (7682), 585-589 (2017).</li>
<li>Jiang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Intestinal+Gastrin%2FCCKBR+%28Cholecystokinin+B+Receptor%29+Ameliorates+Salt-Sensitive+Hypertension+by+Inhibiting+Intestinal+Na%28%2B%29%2FH%28%2B%29+Exchanger+3+Activity+Through+a+PKC+%28Protein+Kinase+C%29-Mediated+NHERF1+and+NHERF2+Pathway%5BTitle%5D)+AND+%22Hypertension%22%5BJournal%5D)">Intestinal Gastrin/CCKBR (Cholecystokinin B Receptor) Ameliorates Salt-Sensitive Hypertension by Inhibiting Intestinal Na(+)/H(+) Exchanger 3 Activity Through a PKC (Protein Kinase C)-Mediated NHERF1 and NHERF2 Pathway.</a> Hypertension. 79 (8), 1668-1679 (2022).</li>
<li>Zheng, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Hypertension+of+liver-yang+hyperactivity+syndrome+induced+by+a+high+salt+diet+by+altering+components+of+the+gut+microbiota+associated+with+the+glutamate%2FGABA-glutamine+cycle%5BTitle%5D)+AND+%22Front+Nutr%22%5BJournal%5D)">Hypertension of liver-yang hyperactivity syndrome induced by a high salt diet by altering components of the gut microbiota associated with the glutamate/GABA-glutamine cycle.</a> Front Nutr. 9, 964273(2022).</li>
<li>Mulligan, S. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multiplexed+TEM+Specimen+Preparation+and+Analysis+of+Plasmonic+Nanoparticles%5BTitle%5D)+AND+%22Microsc+Microanal%22%5BJournal%5D)">Multiplexed TEM Specimen Preparation and Analysis of Plasmonic Nanoparticles.</a> Microsc Microanal. 21 (4), 1017-1025 (2015).</li>
<li>Olson, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Assays+for+Determination+of+Protein+Concentration%5BTitle%5D)+AND+%22Curr+Protoc+Pharmacol%22%5BJournal%5D)">Assays for Determination of Protein Concentration.</a> Curr Protoc Pharmacol. 73, A.3a.1-a.3a.32 (2016).</li>
<li>Matsumoto, H., Haniu, H., Komori, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Determination+of+Protein+Molecular+Weights+on+SDS-PAGE%5BTitle%5D)+AND+%22Methods+Mol+Biol%22%5BJournal%5D)">Determination of Protein Molecular Weights on SDS-PAGE.</a> Methods Mol Biol. 1855, 101-105 (2019).</li>
<li>Sanschagrin, S., Yergeau, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Next-generation+Sequencing+of+16S+Ribosomal+RNA+Gene+Amplicons%5BTitle%5D)+AND+%22J+Vis+Exp%22%5BJournal%5D)">Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons.</a> J Vis Exp. (90), e51709(2014).</li>
<li>Tulkens, J., De Wever, O., Hendrix, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Analyzing+bacterial+extracellular+vesicles+in+human+body+fluids+by+orthogonal+biophysical+separation+and+biochemical+characterization%5BTitle%5D)+AND+%22Nat+Protoc%22%5BJournal%5D)">Analyzing bacterial extracellular vesicles in human body fluids by orthogonal biophysical separation and biochemical characterization.</a> Nat Protoc. 15 (1), 40-67 (2020).</li>
<li>Ruhal, R., Kataria, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Biofilm+patterns+in+gram-positive+and+gram-negative+bacteria%5BTitle%5D)+AND+%22Microbiol+Res%22%5BJournal%5D)">Biofilm patterns in gram-positive and gram-negative bacteria.</a> Microbiol Res. 251, 126829(2021).</li>
<li>Maldonado, R. F., Sá-Correia, I., Valvano, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Lipopolysaccharide+modification+in+Gram-negative+bacteria+during+chronic+infection%5BTitle%5D)+AND+%22FEMS+Microbio+Rev%22%5BJournal%5D)">Lipopolysaccharide modification in Gram-negative bacteria during chronic infection.</a> FEMS Microbio Rev. 40 (4), 480-493 (2016).</li>
<li>Honda, K., Littman, D. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+microbiota+in+adaptive+immune+homeostasis+and+disease%5BTitle%5D)+AND+%22Nature%22%5BJournal%5D)">The microbiota in adaptive immune homeostasis and disease.</a> Nature. 535 (7610), 75-84 (2016).</li>
<li>Guzik, T. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Role+of+the+T+cell+in+the+genesis+of+angiotensin+II+induced+hypertension+and+vascular+dysfunction%5BTitle%5D)+AND+%22J+Exp+Med%22%5BJournal%5D)">Role of the T cell in the genesis of angiotensin II induced hypertension and vascular dysfunction.</a> J Exp Med. 204 (10), 2449-2460 (2007).</li>
<li>Huang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Extracellular+vesicles+as+a+novel+mediator+of+interkingdom+communication%5BTitle%5D)+AND+%22Cytokine+Growth+Factor+Rev%22%5BJournal%5D)">Extracellular vesicles as a novel mediator of interkingdom communication.</a> Cytokine Growth Factor Rev. 73, 173-184 (2023).</li>
<li>Li, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Alleviation+of+colitis+by+honeysuckle+MIR2911+via+direct+regulation+of+gut+microbiota%5BTitle%5D)+AND+%22J+Control+Release%22%5BJournal%5D)">Alleviation of colitis by honeysuckle MIR2911 via direct regulation of gut microbiota.</a> J Control Release. 376, 123-137 (2024).</li>
</ol>

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scheiding en differentiële karakterisering van microbiële extracellulaire blaasjes in de darm bij zoutgevoelige ratten onder zoutrijke dieetomstandigheden

Separation and Differential Characterization of Gut Microbial Extracellular Vesicles in Salt-Sensitive Rats under High-Salt Diet Conditions

Scheiding en differentiële karakterisering van microbiële extracellulaire blaasjes in de darm bij zoutgevoelige ratten onder zoutrijke dieetomstandigheden

High salt intake is a major risk factor for hypertension, and its underlying mechanism may be closely linked to extracellular vesicles (EVs) secreted by ...

separation-differential-characterization-gut-microbial-extracellular

Research

JoVE Journal

Biochemistry

Biochemie

Source: Cian, M. B. et. al., Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii. J. Vis. Exp. (2020)This video demonstrates the separation of the inner and outer membrane fractions of Gram-negative bacteria using sucrose gradient ultracentrifugation. The membrane fractions are purified and stored at low temperatures for targeted analysis of membrane-specific components.

Separation of Inner and Outer Membrane Fractions of Gram-Negative Bacteria

Source: Watson, D. C., et.al. Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria. J. Vis. Exp. (2021)This video demonstrates the isolation and concentration of extracellular vesicles (EVs) derived from the outer membrane of genetically engineered bacteria. The process involves culturing the bacteria to release EVs, followed by sequential centrifugation and filtration steps to remove cells and debris. Finally, ultrafiltration is used to...

Isolation and Enrichment of Outer Membrane-Derived Extracellular Vesicles from Bacteria

Mycobacterium tuberculose vertoont een verhoogde productie en afgifte van extracellulaire blaasjes als reactie op lage ijzer omstandigheden. Dit werk Details een protocol voor het genereren van lage ijzer omstandigheden en methoden voor de zuivering en karakterisering van mycobacteriële extracellulaire blaasjes vrijgegeven in reactie op ijzertekort.

Isolatie en karakterisering van extracellulaire vesicles geproduceerd door ijzer-beperkte mycobacteriën

Bacteriële blaasjes spelen een belangrijke rol bij pathogenese en hebben veelbelovende biotechnologische toepassingen. De heterogeniteit van blaasjes bemoeilijkt analyse en gebruik; daarom is een eenvoudige, reproduceerbare methode nodig om verschillende groottes van blaasjes te scheiden. Hier demonstreren we het gebruik van grootte-uitsluitingschromatografie om heterogene blaasjes geproduceerd door Aggregatibacter actinomycetemcomitanste scheiden .

Grootte uitsluiting chromatografie om bacteriële buitenste membraanblaasje heterogeniteit te analyseren

Bacteriën scheiden nanometergrote extracellulaire blaasjes (EV's) af die bioactieve biologische moleculen dragen. EV-onderzoek richt zich op het begrijpen van hun biogenese, rol in microbe-microbe en gastheer-microbe interacties en ziekte, evenals hun potentiële therapeutische toepassingen. Een workflow voor schaalbare isolatie van EV's van verschillende bacteriën wordt gepresenteerd om standaardisatie van EV-onderzoek te vergemakkelijken.

Schaalbare isolatie en zuivering van extracellulaire blaasjes van Escherichia coli en andere bacteriën

Het huidige protocol biedt gedetailleerde beschrijvingen voor isolatie, concentratie en karakterisering van extracellulaire blaasjes uit cyanobacteriële culturen. Benaderingen voor het zuiveren van blaasjes uit culturen op verschillende schalen, afwegingen tussen methodologieën en overwegingen voor het werken met veldmonsters worden ook besproken.

Isolatie en karakterisering van cyanobacteriële extracellulaire blaasjes

Het doel hier is om een protocol te schetsen om de mechanismen van dysbiose bij hart- en vaatziekten te onderzoeken. Dit artikel bespreekt hoe aseptisch muizenfecale monsters kunnen worden verzameld en getransplanteerd, darmen kunnen worden geïsoleerd en de "Swiss-roll" -methode kan worden gebruikt, gevolgd door immunostainingtechnieken om veranderingen in het maagdarmkanaal te onderzoeken.

Murine fecale isolatie en microbiota transplantatie

Deze studie beschrijft een methode om bacteriële extracellulaire vesikels (BEV's) verrijkt uit menselijke uitwerpselen te isoleren en te zuiveren via dichtheidsgradiëntcentrifugatie (DGC), identificeert de fysieke kenmerken van BEV's op basis van morfologie, deeltjesgrootte en concentratie, en bespreekt de mogelijke toepassingen van de DGC-benadering in klinisch en wetenschappelijk onderzoek.

Isolatie en zuivering van bacteriële extracellulaire blaasjes uit menselijke uitwerpselen met behulp van dichtheidsgradiëntcentrifugatie

Dit artikel beschrijft: (1) orale gavageafgifte van testmoleculen aan neonatale muizen al vanaf DOL 6; en (2) ex vivo evaluatie van de functie van colonische epitheelbarrières in biopten van DOL 10 tot spenende (DOL 20) jongen door het beoordelen van paracellulaire en transcellulaire permeabiliteit met Ussing-kamers.

Orale Gavage bij neonatale muispups en functionele beoordeling van de integriteit van darmbarrières met behulp van Ussing-kamers

Gut host-microbe interacties werden geschat aan de hand van een nieuwe benadering van het combineren van een synthetische mondelinge Gemeenschap, in vitro gastro-intestinale spijsvertering en een model van het epitheel van de dunne darm. Presenteren we een methode die aangepast worden kan voor de beoordeling van de cel invasie van ziekteverwekkers en multispecifieke biofilms, of zelfs om te testen op het overlevingsvermogen van probiotische formuleringen.

Beoordelen van de levensvatbaarheid van een synthetische bacteriële Consortium op de In Vitro -Interface van het Gut-Host-microbe

Leer hoe je microbiële extracellulaire blaasjes isoleert uit de darm van zoutgevoelige ratten met dichtheidsgradiëntcentrifugatie. Deze methode karakteriseert grootte, morfologie en samenstelling voor hypertensieonderzoek.

Scheiding en differentiële karakterisering van microbiële extracellulaire blaasjes in de darm bij zoutgevoelige ratten onder zoutrijke dieetomstandigheden (Video) | JoVE Journal

Scheiding en differentiële karakterisering van microbiële extracellulaire blaasjes in de darm bij zoutgevoelige ratten onder zoutrijke dieetomstandigheden

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