432 wyświetleń
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1 lipca 2016
A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure.
The Final Concentration of EGTA in Solution 2 and the Final Concentration of Calcium chloride dihydrate in solutions 3 and 4 have been updated in Table 1.
A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
The changes listed below have been made to Table 1.
1). In the recipe for Solution 2, the Final Concentration of EGTA has been changed from:
| EGTA | 0.1mM |
to:
| EGTA | 1mM |
2). In the recipes for Solution 3 and 4, the Final Concentration of Calcium chloride dihydrate has been changed from:
| Calcium chloride dihydrate | 0.5µM |
to:
| Calcium chloride dihydrate | 5mM |
A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
The changes listed below have been made to Table 1.
1). In the recipe for Solution 2, the Final Concentration of EGTA has been changed from:
| EGTA | 0.1mM |
to:
| EGTA | 1mM |
2). In the recipes for Solution 3 and 4, the Final Concentration of Calcium chloride dihydrate has been changed from:
| Calcium chloride dihydrate | 0.5µM |
to:
| Calcium chloride dihydrate | 5mM |