Organotypic Model
Organotypic Model - Related Videos
07:48
Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells
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Cited by 18 •
2017
Organotypic hippocampal slice cultures (OHSC) represent an in vitro model that simulates the in vivo situation very well. Here we describe a vibratome-based improved slicing protocol to obtain high quality slices for use in assessing the neuroprotective potential of novel substances or the biological behavior of tumor cells.
03:53
Laser Microdissection: A Technique to Isolate Invading Colorectal Cancer cells from a 3D Organotypic Culture Model
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2023
The video describes the laser dissection of the 3D organotypic co-culture model with micron-level precision. In 3D organotypic models, stromal constituents such as fibroblasts are 3-dimensionally co-cultured with cancer epithelial cells. The 3D model serves as an experimental tool that enables invasion and cancer-stroma interactions to be studied in near-physiological conditions.
09:00
Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity
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Cited by 20 •
2015
Here, we present a protocol to construct a three-dimensional in vitro model of the lining of the peritoneal cavity, composed of primary human mesothelial cells and fibroblasts layered with extracellular matrix, as a tool to investigate ovarian cancer cell adhesion, invasion, and proliferation.
08:54
Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity
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Cited by 20 •
2016
Cells growing in a three-dimensional (3-D) environment represent a marked improvement over cell cultivation in 2-D environments (e.g., flasks or dishes). Here we describe the development of a multicellular 3-D organotypic model of the human intestinal mucosa cultured under microgravity provided by rotating-wall-vessel (RWV) bioreactors.
04:07
Generation of Organotypic Full Skin Reconstructs: A Procedure to Establish 3D Skin Model Using Human Skin Samples
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2023
This video describes the detailed protocol for generating in vitro 3D organotypic skin reconstructs to study melanoma progression. This skin model mimics both the architecture and multicellular complexity of organs in vivo.
FAQs
An organotypic model preserves three-dimensional tissue architecture and enables interactions among neighboring cells, rather than examining cells in isolation. These relationships can influence how cells organize, communicate, and respond to experimental conditions. As a result, the system can reveal tissue-level behavior that may be missed when cells are studied in a simplified culture environment.
The extracellular matrix or biomaterial scaffold provides a supportive environment in which cells can organize and develop tissue-like characteristics. Its presence helps maintain spatial relationships and supports interactions between cells and their surroundings. In bioengineering studies, comparing scaffold-based environments can help evaluate how material design affects biological function and the development of engineered tissue.
Primary cells, stem-cell-derived cells, and tissue fragments can provide different starting points for reproducing organ or tissue behavior. The selected source influences which cellular interactions and functional characteristics the model can develop. This choice should therefore match the biological question, whether the goal is to study development, disease mechanisms, regeneration, or responses to an intervention.
Controlled laboratory conditions allow researchers to examine tissue responses while limiting variation from the surrounding environment. Within those conditions, organized cells and supporting materials can be observed as tissue-like behavior develops. This makes it possible to compare responses to drugs, biomaterials, or other experimental factors and to connect observed outcomes with specific changes in the engineered system.
Construction begins by selecting appropriate primary cells, stem-cell-derived cells, or tissue fragments, then placing them within an extracellular matrix or biomaterial scaffold. The assembled system is maintained under controlled conditions so cell-cell interactions, architecture, and functional responses can develop. Researchers can then examine how the resulting tissue-like system responds to defined experimental factors.
Researchers may choose this approach when simplified cell cultures do not adequately represent tissue-level behavior. Its organized cellular environment supports assessment of responses to drugs or biomaterials in a setting that more closely reflects tissue structure and function. In bioengineering, these results can inform material design, therapeutic investigation, and efforts to create engineered tissues with improved biological function.
The same general platform can be adapted to different biological questions by selecting relevant cells or tissue fragments and examining how organization and function change under controlled conditions. Development studies can focus on tissue formation, disease studies on altered tissue behavior, and regeneration studies on restoration or improvement of biological function. This flexibility makes the models useful across bioengineering research.
An organotypic model can provide information about coordinated tissue behavior, including the relationship between cellular organization, tissue architecture, and functional responses. These outcomes help researchers judge whether a drug or biomaterial supports or disrupts biological function in a structured system. The resulting evidence can also guide the design of engineered tissues intended to perform more effectively.