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Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity
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JoVE Journal Medicine
Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

DOI:
10.3791/53541-v

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09:00 min

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December 31, 2015

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, , ,
1Department of Obstetrics and Gynecology, Section of Gynecologic Oncology,University of Chicago

Chapters

  • 00:05Title
  • 01:00Primary Human Mesothelial Cell (HPMC) Isolation and Culture
  • 03:57Normal Omentum Fibroblast (NOF) Isolation and Culture
  • 04:54Organotypic Culture Plating
  • 06:47Results: The Role of Integrins in Ovarian Cancer Cell Adherence, Proliferation and Invasion
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to construct a three-dimensional in vitro model of the lining of the peritoneal cavity, composed of primary human mesothelial cells and fibroblasts layered with extracellular matrix, as a tool to investigate ovarian cancer cell adhesion, invasion, and proliferation.

Tags

Keywords: Ovarian CancerMetastasisPeritoneal CavityOrganotypic CultureFibroblastsMesothelial CellsExtracellular MatrixAdhesionInvasionProliferationMicroenvironmentHigh-throughput ScreeningMetastasis Inhibitors

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