Microscopic Spermatozoa Evaluation

Microscopic spermatozoa evaluation is a laboratory technique for examining sperm cells in semen to assess their number, movement, and structural characteristics, providing important information about male reproductive function. In this method, a prepared semen sample is viewed under a microscope, allowing analysts to estimate sperm concentration, classify motility patterns, and identify morphological features such as head, midpiece, and tail abnormalities. The results support fertility investigations, reproductive biology research, assisted reproduction, and studies of environmental or treatment-related effects on sperm quality. Standardized sample handling, timing, and observation conditions are essential for producing reliable and comparable measurements.

Microscopic Spermatozoa Evaluation - Related Videos

Research

JoVE Journal - Developmental Biology

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa

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Cited by 8 •

2018

This protocol describes a detailed methodology for detecting H2O2 localization within Solea senegalensis spermatozoa using a sensitive fluorochrome DCFH-DA for ROS, a live mitochondria stain for mitochondria, and DAPI for nuclei visualization, respectively. The protocol is designed to be performed within 2 h with either fresh or thawed spermatozoa.

A Technique for Percutaneous Epididymal Spermatozoa Aspiration and Processing

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2026

Source:Li Xiaoming1, Liu Xiao1, Li Haiyang1, Cai Xiaohua1, Ou Jianping11Center for Reproductive Medicine, The Third Affiliated Hospital of Sun Yat-sen University.This video demonstrates the percutaneous aspiration of epididymal spermatozoa from a patient with azoospermia, a condition characterized by the absence of spermatozoa in the semen. The procedure involves disinfecting the scrotal area, aspirating epididymal fluid using a syringe with a specialized culture solution and processing the...

Research

JoVE Journal - Biology
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Phosphopeptide Analysis of Rodent Epididymal Spermatozoa

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Cited by 26 •

2014

Proteomic analysis of any cell type is highly dependent on both purity and pre-fractionation of the starting material in order to de-complexify the sample prior to liquid chromatography mass spectrometry (MS). By using back-flushing techniques, pure spermatozoa can be obtained from rodents. Following digestion, phosphopeptides can be enriched using TiO2.

Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization

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Cited by 8 •

2017

Here, we present protocols that have been successfully used for dolphin spermatozoa collection, cryopreservation, and heterologous IVF performance using bovine oocytes.

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

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Cited by 5 •

2018

Here we present protocols for novice researchers to initiate phenotyping for the pharmacologically important bacterial genus Streptomyces.

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