Cfse-labeled Tumor Complex
Cfse-labeled Tumor Complex - Related Videos
09:49
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
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Cited by 39 •
2010
Many infections elicit a strong CTL response, but occasionally, the quantity of responding cells does not correlate to control of the pathogen1. One measure of CTL quality is their ability to kill specifically2. CFSE labeling of target cells can be used to investigate this CTL response quality in vivo3,4.
07:14
A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with Mycobacterium bovis Bacille Calmette-Guérin
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Cited by 10 •
2016
An assay in mice to track cell migration from skin to draining lymph node is described which enables the characterization of skin Dendritic cells mobilized to the lymph node after footpad infection with Bacille Calmette-Guérin.
04:10
The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation
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Cited by 214 •
2010
CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein. As such, when a CFSE-labeled cell divides, its progeny have half the amount of fluorescence, which can thereby be used to assess cell division. This article describes the procedures typically used for labeling mouse lymphocytes with CFSE.
11:54
Identification and Isolation of Slow-Dividing Cells in Human Glioblastoma Using Carboxy Fluorescein Succinimidyl Ester (CFSE)
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Cited by 18 •
2012
This video protocol demonstrates the application of the fluorescent dye carboxyfluorescein succinimidyl ester (CFSE) for the identification and separation of different sub-populations of cells in human glioblastoma based on frequency of cell division.
07:23
A Protocol for Genetic Induction and Visualization of Benign and Invasive Tumors in Cephalic Complexes of Drosophila melanogaster
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Cited by 5 •
2013
Cooperation between an activated oncogene like RASV12 and mutations in cell polarity genes like scribbled, result in tumor growth in Drosophila where tumor cells also display invasive behaviors. Here a simple protocol for the induction and observation of the benign and invasive tumors is presented.
FAQs
The key chemical feature is CFSE’s reaction with intracellular amines, which creates stable covalent bonds rather than a transient association. Because the tracer remains attached within labeled tumor cells, fluorescence can serve as a continuing identifier while those cells are followed through an experimental system. This chemical stability supports measurements of persistence and location alongside proliferation.
During cell division, fluorescence is approximately divided between daughter cells. Researchers can therefore interpret progressively distributed fluorescence as evidence related to tumor-cell proliferation, rather than treating one intensity measurement as a complete growth readout. This division-linked behavior is useful for connecting detectable signal changes with tumor-cell behavior over the course of an experiment.
Persistence is assessed by whether labeled tumor cells remain detectable, whereas division changes how fluorescence is distributed among daughter cells. Examining these patterns together can help separate continued presence from proliferation-related signal changes. That distinction matters when interpreting whether an experimental condition influences tumor-cell persistence, expansion, or both within a complex system.
Within complex experimental systems, the fluorescently marked tumor-cell population provides a way to track where tumor cells are while examining their behavior in relation to other cells. Changes in detectable distribution can be considered alongside interaction-focused observations, allowing studies to connect tumor-cell location and persistence with cell-cell interactions rather than analyzing growth in isolation.
A basic workflow begins with a tumor-cell preparation marked using CFSE, followed by placement or observation of that labeled population within the relevant experimental system. Researchers then use fluorescence-based analysis to follow detectable signal patterns. Interpreting those patterns can provide information about tumor-cell proliferation, persistence, distribution, and behavior under the selected experimental conditions.
Fluorescence patterns allow researchers to examine how labeled tumor cells behave in the presence of anticancer treatments. Treatment-associated changes in proliferation, persistence, or distribution can be linked to changes in the tumor-cell population. This makes the approach useful for studying treatment responses while retaining information about tumor-cell behavior within a complex experimental setting.