Orthotopic Allograft Implantation
Orthotopic Allograft Implantation - Related Videos
05:10
Intracranial Orthotopic Allografting of Medulloblastoma Cells in Immunocompromised Mice
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Cited by 5 •
2010
This protocol describes the isolation and dissociation of mouse medulloblastoma tissue, and subsequent allografting of the tumor cells into immunocompromised recipient mice in order to initiate secondary medulloblastoma.
06:20
Syngeneic Mouse Orthotopic Allografts to Model Pancreatic Cancer
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Cited by 4 •
2022
Syngeneic mouse orthotopic allografts of pancreatic ductal adenocarcinoma (PDAC) recapitulate the biology, phenotypes, and therapeutic responses of disease subtypes. Owing to their fast, reproducible tumor progression, they are widely used in preclinical studies. Here, we show common practices to generate these models, injecting syngeneic murine PDAC cultures into the pancreas.
06:59
A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection
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Cited by 2 •
2022
The rat orthotopic renal transplantation model contributes to investigating the mechanism of renal allograft rejection. The current model increases the recipients' survival without interference with blood supply and venous reflux of the lower body using an end-to-end anastomosis of kidney implantation and an end-to-side "tunnel" method of ureter-bladder anastomosis.
07:15
Orthotopic Hind Limb Transplantation in the Mouse
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Cited by 24 •
2016
This novel model for orthotopic hind limb transplantation in the mouse, applying a non-suture cuff technique for super-microvascular anastomosis, provides a powerful tool for in vivo mechanistic immunological research related to vascularized composite allotransplantation (VCA).
05:08
Fluorescent Orthotopic Mouse Model: Implanting GFP Expressing Cancer Cells into Mouse for Assessing Tumor Progression In Vivo
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2023
This video demonstrates the protocol for implanting green fluorescent protein-expressing pancreatic cancer cells orthotopically into the pancreas of the mouse. The orthotopic mouse model helps in investigating the efficacy of novel therapeutics on primary and metastatic tumors.
FAQs
Placing the graft in the matching organ preserves the tissue context that can shape tumor behavior. Local stromal cells, blood vessels, and extracellular matrix interact with the implanted tumor, influencing growth and dissemination. Consequently, investigators can study cancer progression and invasion under conditions that more closely reflect the tumor’s native anatomical setting.
Host immunity can become part of the model when the recipient immune system is applicable to the experimental design. Immune interactions may influence tumor growth and dissemination alongside signals from the local tissue. Accounting for this component helps researchers interpret whether an observed cancer outcome reflects tumor behavior alone or its interaction with the host environment.
Its placement within the relevant organ supports analysis of tumor progression, invasion, and metastasis as connected outcomes rather than as isolated growth measurements. Because the graft encounters local stromal cells, blood vessels, and extracellular matrix, the model can help investigators examine how the native tissue setting shapes tumor dissemination.
A basic workflow includes obtaining tumor cells or tissue from a donor of the same species, selecting the recipient’s corresponding organ, and surgically positioning the graft within that organ. This sequence establishes the intended anatomical relationship before investigators assess tumor behavior. The essential design feature is placement in the relevant organ rather than elsewhere in the recipient.
Researchers choose this approach when they need a cancer model that preserves organ-specific surroundings while examining disease mechanisms. The resulting setting is useful for studying tumor progression, invasion, and metastasis because local stromal components, vessels, and extracellular matrix remain relevant to the implanted tissue. It therefore supports questions requiring physiologically relevant tumor behavior.
Treatment studies can use the model to evaluate how tumors respond when situated in their relevant organ and exposed to host and tissue influences. Investigators can examine treatment-associated effects on tumor growth, progression, or dissemination alongside underlying disease mechanisms. This makes orthotopic allograft implantation useful for assessing therapeutic strategies in a preclinical cancer research setting.