Biodegradable Nanoparticles
Biodegradable Nanoparticles - Related Videos
09:01
Programming Stem Cells for Therapeutic Angiogenesis Using Biodegradable Polymeric Nanoparticles
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Cited by 13 •
2013
We describe the method of programming stem cells to overexpress therapeutic factors for angiogenesis using biodegradable polymeric nanoparticles. Processes described include polymer synthesis, transfecting adipose-derived stem cells in vitro, and validating the efficacy of programmed stem cells to promote angiogenesis in a murine hindlimb ischemia model.
11:42
Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers
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Cited by 3 •
2019
Crystallization-driven self-assembly (CDSA) displays the unique ability to fabricate cylindrical nanostructures of narrow length distributions. The organocatalyzed ring-opening polymerization of ε-caprolactone and subsequent chain extensions of methyl methacrylate and N,N-dimethyl acrylamide are demonstrated. A living CDSA protocol that produces monodisperse cylinders up to 500 nm in length is outlined.
08:13
Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles
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Cited by 1 •
2021
Iron oxide nanoparticles are synthesized via a nonaqueous sol gel procedure and coated with anionic short molecules or polymer. The use of magnetometry for monitoring the incorporation and biotransformations of magnetic nanoparticles inside human stem cells is demonstrated using a vibrating sample magnetometer (VSM).
08:51
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
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Cited by 9 •
2013
A protocol for nanoparticle tracking analysis (NTA) and high-throughput flow cytometry to evaluate polymeric gene delivery nanoparticles is described. NTA is utilized to characterize the nanoparticle particle size distribution and the plasmid per particle distribution. High-throughput flow cytometry enables quantitative transfection efficacy evaluation for a library of gene delivery biomaterials.
08:43
Aerobic Biodegradation Testing of Materials Using a Natural Marine Seawater Inoculum and Closed Loop Respirometer
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2025
The protocol describes standard operating procedure for testing potentially biodegradable materials both synthetic and natural under aerobic conditions using a natural seawater inoculum and automated closed loop respirometry system.
FAQs
The release profile depends on how quickly the particle matrix changes under physiological conditions. Hydrolysis, enzymatic degradation, or related processes progressively erode the matrix, allowing an encapsulated or surface-bound agent to become available over time. This relationship between matrix breakdown and payload release is important when researchers assess whether delivery is controlled and therapeutically useful.
Payload placement affects how researchers interpret release behavior. An encapsulated agent is held within the particle matrix, whereas a surface-bound agent is associated with the particle exterior. Because these arrangements expose the payload to the surrounding physiological environment differently, investigators examine them when studying delivery, degradation, and the resulting availability of therapeutic or diagnostic agents.
Biodegradability matters because particles intended for clinical use must be considered not only for delivery performance but also for what happens after their function. Researchers therefore examine matrix breakdown alongside biocompatibility and clearance. A design that supports useful release while potentially limiting long-term accumulation may offer a more favorable safety profile, although performance must be evaluated experimentally.
Evaluation focuses on interconnected outcomes rather than a single measurement. Clinical research examines degradation behavior, release of the therapeutic or diagnostic payload, biocompatibility, clearance, and tissue distribution. Together, these observations help determine whether the particles provide the intended delivery or imaging function and whether their breakdown and removal are compatible with safe use.
Researchers investigate these carriers when a therapeutic or diagnostic agent needs improved solubility, stability, or tissue distribution. The same platform is studied for targeted drug delivery, imaging, vaccine formulation, and treatment strategies. These applications reflect different performance goals, so studies must connect particle behavior with the specific payload and clinical purpose being examined.
In vaccine formulation, imaging, and drug-delivery studies, the particles provide a way to transport an agent while their matrix gradually changes in the body. Investigators can then examine whether the system improves distribution, maintains useful stability, or supports delivery to a desired tissue. The relevant outcome depends on the application and the payload.