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JoVE Journal
Bioengineering
Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically ...
Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically ...
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

Using Magnetometry to Monitor Cellular Incorporation and Subsequent Biodegradation of Chemically Synthetized Iron Oxide Nanoparticles

Full Text
5,087 Views
08:13 min
February 27, 2021

DOI: 10.3791/61106-v

Aurore Van de Walle1, Anouchka Plan Sangnier1,2, Alexandre Fromain1, Claire Wilhelm1, Yoann Lalatonne2,3

1Laboratoire Matière et Systèmes,Complexes MSC, UMR 7057, CNRS & University Paris Diderot, 2Université Sorbonne Paris Nord, Laboratory for Vascular Translational Science,LVTS, INSERM, UMR 1148, 3Services de Biochimie et Médecine Nucléaire,Hôpital Avicenne Assistance Publique-Hôpitaux de Paris

Iron oxide nanoparticles are synthesized via a nonaqueous sol gel procedure and coated with anionic short molecules or polymer. The use of magnetometry for monitoring the incorporation and biotransformations of magnetic nanoparticles inside human stem cells is demonstrated using a vibrating sample magnetometer (VSM).

This video introduces a fast and efficient method for the synthesis of magnetic nanoparticles and the use of magnetometry to photograph transformation in the biological environment. Magnetic nanoparticles have attracted increased attention for biomedical applications, And it is important to understand that fate once internalized in cells. This is achieved here by measuring the cellular magnetism.

For both magnetic nanoparticles synthesis and measurements, result could be affected by contamination. Use well-cleaned chemical vials and make sure that the magnetometer equipment is free of contamination. The magnetometry samples holder is usually used for powder samples.

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