Post-surgical Infections
Post-surgical Infections - Related Videos
08:33
Surgical Transplantation of Mouse Neural Stem Cells into the Spinal Cords of Mice Infected with Neurotropic Mouse Hepatitis Virus
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Cited by 14 •
2011
The transplantation of mouse neural stem cells (NSCs) into the spinal cords of mice with established demyelination is detailed. The preparation of NSCs, the laminectomy of thoracic vertebra 9 (T9), and transplantation of NSCs is outlined along with the pre- and post-operative care of the mice.
11:18
Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens
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Cited by 234 •
2012
Transparent zebrafish embryos have proved useful model hosts to visualize and functionally study interactions between innate immune cells and intracellular bacterial pathogens, such as Salmonella typhimurium and Mycobacterium marinum. Micro-injection of bacteria and multi-color fluorescence imaging are essential techniques involved in the application of zebrafish embryo infection models.
12:30
Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection
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Cited by 108 •
2013
The larva of the wax moth Galleria mellonella was recently established as an in vivo model to study Legionella pneumophila infection. Here, we demonstrate fundamental techniques to characterize the pathogenesis of Legionella in the larvae, including inoculation, measurement of bacterial virulence and replication as well as extraction and analysis of infected hemocytes.
12:38
4D Multimodality Imaging of Citrobacter rodentium Infections in Mice
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Cited by 19 •
2013
Multi-modality imaging is a valuable approach for studying bacterial colonization in small animal models. This protocol outlines infection of mice with bioluminescent Citrobacter rodentium and the longitudinal monitoring of bacterial colonization using composite 3D diffuse light imaging tomography with μCT imaging to create a 4D movie of C. rodentium infection.
07:59
Bioluminescence Imaging to Detect Late Stage Infection of African Trypanosomiasis
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Cited by 3 •
2016
This manuscript describes the use of a bioluminescent strain of African trypanosomes to enable the tracking of late stage infection and demonstrates how in vivo live imaging can be used to visualize infections within the central nervous system in real-time.
FAQs
Tissue injury can weaken local defenses that normally limit microbial growth. When blood flow is impaired, healing tissues may receive less support, while accumulated fluid can create a favorable environment for microorganisms. These conditions allow introduced microbes to persist and multiply more readily, increasing the chance that inflammation will interfere with normal repair and recovery.
Fluid accumulation can support microbial growth at or near the operative site, particularly when surrounding tissue is already damaged or poorly perfused. Implanted materials may also provide a surface or foreign environment that helps microorganisms remain associated with the site. Together, these factors can make local infection harder to control and may threaten surgical healing.
These findings can reflect inflammation and immune activity responding to microorganisms at the operative site. Their presence may signal involvement of skin or soft tissue, while worsening local findings or systemic features can raise concern for deeper or more extensive infection. Clinical interpretation therefore requires examination rather than relying on one symptom in isolation.
Assessment begins with clinical examination of the operative area and consideration of symptoms affecting recovery. Clinicians evaluate local findings such as pain, redness, or drainage together with broader signs such as fever. Appropriate laboratory testing can add information about the suspected infection and help connect observed findings with decisions about further management.
Laboratory testing complements examination by providing additional evidence about the infectious process. Its results can help guide treatment rather than relying only on visible changes at the surgical site. Used with clinical assessment, testing supports decisions intended to control infection, protect the operation’s outcome, and reduce the likelihood that local disease will progress.
Managing these infections promptly and appropriately can reduce complications, support tissue healing, and protect the intended surgical result. Preventive and therapeutic decisions are informed by examination and laboratory findings, allowing clinicians to address infection before it interferes further with recovery. This approach also aims to limit progression from a local problem to deeper or systemic involvement.