Progeny Testing Strategy
Progeny Testing Strategy - Related Videos
08:39
Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing
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Cited by 8 •
2017
The goal of this protocol is to alter the penetrance of lethal skeletal mutant phenotypes in zebrafish by selective breeding. Lethal mutants cannot be grown to adulthood and bred themselves, therefore this protocol describes a method for tracking and selecting penetrance through multiple generations by progeny testing.
12:59
Barnes Maze Testing Strategies with Small and Large Rodent Models
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Cited by 171 •
2014
The dry-land Barnes maze is widely used to measure spatial navigation ability in response to mildly aversive stimuli. Over consecutive days, performance (e.g. latency to locate escape cage) of control subjects improves, indicative of normal learning and memory. Differences between rats and mice necessitate apparatus and methodology changes that are detailed here.
08:36
Modified ES / OP9 Co-Culture Protocol Provides Enhanced Characterization of Hematopoietic Progeny
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Cited by 12 •
2011
mStrawberry OP9 cells allow for complete evaluation of all ES-derived progeny from co-culture.
08:59
Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry
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Cited by 13 •
2017
While infiltrating macrophages are continuously recruited to adult tissues from circulating precursors, resident macrophages seed their tissue during development, where they are maintained without further input from progenitors. The progenitors for resident macrophages were recently identified. Here, we present methods for the genetic fate mapping of the resident macrophage progenitors.
09:45
New Variations for Strategy Set-shifting in the Rat
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Cited by 3 •
2017
Set-shifting, a form of behavioral flexibility, requires an attentional shift from one stimulus dimension to another. We extended an established rodent set-shifting task1 by requiring attention to different stimuli according to context. The task was combined with specific lesions to identify neuron subtypes underlying a successful shift.
FAQs
Comparing offspring from different families under consistent conditions helps researchers determine whether a developmental trait follows parental inheritance rather than differences in surroundings. Measuring the same phenotype or genotype across families provides a basis for relating variation in embryonic development, growth, morphology, or fertility to parental alleles, while reducing the influence of environmental differences on the observed outcome.
A recessive mutation may remain unrecognized in a candidate parent when its visible effects are absent or limited. Examination of offspring phenotypes or genotypes can expose the mutation through inherited trait patterns across families. This makes progeny testing strategy useful for identifying hidden genetic variation relevant to developmental abnormalities, fertility, or disease-related models.
Selected partners create defined family comparisons that help researchers examine how parental alleles are transmitted and expressed in offspring. When offspring are assessed across these families, recurring phenotypic or genotypic patterns can provide stronger evidence for a parental contribution than observations from a single mating. The approach therefore connects family variation with developmental outcomes.
The process begins by choosing a candidate parent and selected mating partners, followed by producing offspring families. Researchers then measure relevant offspring phenotypes or genotypes, ideally under consistent conditions, and compare results across families. Depending on the study, measurements may focus on embryonic development, growth, morphology, fertility, or other traits linked to inherited variation.
Applications can include traits expressed during embryonic development, postembryonic growth, body morphology, and fertility. Researchers may examine these characteristics as phenotypes or investigate corresponding genotypes in offspring. Evaluating several trait types allows the same breeding framework to connect parental genetic variation with developmental processes and to identify lines that show biologically important differences.
Results can reveal genetic variation that distinguishes families or breeding lines in developmental and disease-related traits. Researchers can use this information to identify organisms with informative inherited phenotypes, including lines carrying recessive mutations or showing consistent differences in development, morphology, growth, or fertility. Such comparisons help align model selection with the goals of a developmental biology study.