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Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry
DOI:

08:59 min

July 17, 2017

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Chapters

  • 00:05Title
  • 01:01Dissection of the Yolk Sac and Fetal Liver
  • 03:41Processing of Embryonic Tissues for Flow Cytometry
  • 05:18Surface Antigen Staining
  • 06:37Results: Identification of Ertythromyeloid Progentitor Cells and Yolk Sac-derived Macrophages
  • 08:14Conclusion

Summary

Automatic Translation

While infiltrating macrophages are continuously recruited to adult tissues from circulating precursors, resident macrophages seed their tissue during development, where they are maintained without further input from progenitors. The progenitors for resident macrophages were recently identified. Here, we present methods for the genetic fate mapping of the resident macrophage progenitors.

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