Kir Gene Typing
Kir Gene Typing - Related Videos
10:06
Cell Type-specific Gene Expression Profiling in the Mouse Liver
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Cited by 1 •
2019
Translating ribosome affinity purification (TRAP) enables rapid and efficient isolation of cell type-specific translating mRNA. Here, we demonstrate a method that combines hydrodynamic tail-vein injection in a mouse model of liver repopulation and TRAP to examine the expression profile of repopulating hepatocytes.
08:37
Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
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Cited by 17 •
2015
The goal of this protocol is to use the fluorescence activated cell sorting (FACS) technique to sort specific types of neural cells for subsequent analysis of cell-type-specific gene expression, epigenetic markers, and or protein expression.
02:18
Types of Genetic Transfer Between Organisms
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2020
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
07:58
qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
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Cited by 8 •
2019
Quantitative killer cell immunoglobulin-like receptor (KIR) semi-automated typing (qKAT) is a simple, high-throughput, and cost-effective method to copy number type KIR genes for their application in population and disease association studies.
12:55
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
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Cited by 11 •
2010
This procedure shows how to use the Gene Pulser MXcell electroporation system to rapidly and easily identify the best electroporation conditions for mouse embryonic fibroblasts (MEFs) or other primary cells. Considerations for troubleshooting are also discussed in the associated video.
FAQs
Different combinations of KIR genes define which receptor components may be encoded in an individual’s genome. Because these receptors regulate natural killer cell and T-cell responses, comparing gene content helps investigators examine variation in immune-cell recognition. This genetic perspective can clarify how receptor repertoires relate to host-pathogen interactions, disease susceptibility, or other immune outcomes.
Gene-content analysis determines whether particular KIR genes are present or absent in genomic DNA, whereas allele-focused analysis examines sequence differences within an identified gene. These measurements answer different genetic questions: presence or absence describes the receptor repertoire, while allelic resolution provides finer information about variants. Studies select the level of resolution according to their research objective.
KIR haplotypes summarize combinations of KIR genes inherited together and therefore add genomic context beyond isolated gene calls. Researchers can compare haplotypic patterns among individuals or study groups to investigate links with immune regulation and disease-related traits. Haplotypic information is also relevant when assessing genetic compatibility and immune responses in transplantation research.
Targeted polymerase chain reaction can support assays that determine whether selected KIR genes are present or absent, making it useful for gene-content characterization. Sequencing can be applied when the study needs to distinguish allelic variants at the DNA level. The appropriate approach depends on whether the research question concerns broad gene content or finer genetic resolution.
A typical workflow begins with genomic DNA, followed by targeted molecular testing of selected KIR loci. The resulting assay data are interpreted to identify gene presence or absence and, when required, sequence-level allelic differences. Researchers can then organize these calls into gene-content profiles or haplotypic patterns for comparison with immune, clinical, or genetic study variables.
Typing provides donor and recipient genetic information about KIR gene content and haplotypes, which can be examined in relation to donor-recipient compatibility. In transplantation research, these profiles help investigators study how inherited receptor variation may relate to immune responses after hematopoietic stem-cell transplantation. The method therefore contributes genetic context to analyses of post-transplant immune biology.
KIR gene typing is used in investigations of host-pathogen interactions, reproductive immunology, and disease susceptibility. In each setting, researchers compare receptor gene content or allelic patterns with the biological or clinical feature under study. This makes the approach useful for connecting inherited variation in immune-regulatory receptors with differences in immune response across populations or conditions.