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qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
JoVE Journal
Genetics
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JoVE Journal Genetics
qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

DOI:
10.3791/58646-v

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07:58 min

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March 06, 2019

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, , , , ,
1Department of Pathology,University of Cambridge, 2Department of Physiology, Development and Neuroscience,University of Cambridge, 3Department of Obstetrics and Gynaecology,University of Cambridge School of Medicine, NIHR Cambridge Biomedical Research Centre, 4Centre for Trophoblast Research,University of Cambridge, 5Department of Genetics & Evolution,University of Geneva, 6Royal Papworth Hospital, 7Department of Plant Sciences,University of Cambridge

Chapters

  • 00:04Title
  • 01:45Preparation and Plating Out of DNA
  • 02:25Preparation of the Master Mix
  • 03:36qPCR Assay
  • 04:07Post-run Analysis
  • 05:19Export of Results and Copy Number Calculations
  • 06:39Results: qKAT Used to Copy Number Type KIR Genes
  • 06:59Conclusion

Summary

Automatic Translation

Automatic Translation

Quantitative killer cell immunoglobulin-like receptor (KIR) semi-automated typing (qKAT) is a simple, high-throughput, and cost-effective method to copy number type KIR genes for their application in population and disease association studies.

Tags

Keywords: QKATQuantitative KIR Automated TypingKiller-cell Immunoglobulin-like ReceptorNK Cell Receptor GeneticsHaplotype DiversityDisease AssociationHigh-throughputGene Copy NumberMultiplex PCRReal-time PCRRelative QuantificationPopulation GeneticsHLAViral InfectionsCancerStem Cell TransplantationPregnancy Disorders

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