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Cell Type-specific Gene Expression Profiling in the Mouse Liver
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Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell Type-specific Gene Expression Profiling in the Mouse Liver

DOI:
10.3791/60242-v

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10:06 min

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September 17, 2019

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, ,
1Department of Genetics,University of Pennsylvania, 2Department of Medicine,University of Pennsylvania

Chapters

  • 00:04Title
  • 01:21Conjugation of Antibodies to Magnetic Beads
  • 03:36Liver Tissue Lysis
  • 05:33Immunoprecipitation and RNA Isolation
  • 07:37Results: Cell Type-specific Isolation of High-quality RNA by Trap
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

Translating ribosome affinity purification (TRAP) enables rapid and efficient isolation of cell type-specific translating mRNA. Here, we demonstrate a method that combines hydrodynamic tail-vein injection in a mouse model of liver repopulation and TRAP to examine the expression profile of repopulating hepatocytes.

Tags

Keywords: TRAP-seqCell Type-specificGene ExpressionLiverHepatocytesLiver InjuryCell TypesMagnetic BeadsProtein LAntibody

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